BD OptEIATM Human TGF-beta1 ELISA Set

Description

This standard curve is fordemonstration only. A standard curve must be run with each assay. Typical Standard Curve and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing. Serial dilutions within the plate may also be performed by pipetting 100 muL of Assay Diluent into each standard well except the highest 8000 pg/mL , then adding 100 muL of the 8000 pg/mL standard to both that well and the 4000 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 muL of the 4000 pg/mL standard to the 2000 pg/mL well. Continue these dilutions to the 125 pg/mL standard well, out of which the extra 100 muL should be discarded . This standard curve is fordemonstration only. A standard curve must be run with each assay. Typical Standard Curve and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing. Show More Serial dilutions within the plate may also be performed by pipetting 100 muL of Assay Diluent into each standard well except the highest 8000 pg/mL , then adding 100 muL of the 8000 pg/mL standard to both that well and the 4000 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 muL of the 4000 pg/mL standard to the 2000 pg/mL well. Continue these dilutions to the 125 pg/mL standard well, out of which the extra 100 muL should be discarded . Show More This standard curve is fordemonstration only. A standard curve must be run with each assay. Typical Standard Curve and 20-plate yield were obtained in the BD Biosciences Pharmingen laboratory, using the recommended procedure and manual plate washing. Serial dilutions within the plate may also be performed by pipetting 100 muL of Assay Diluent into each standard well except the highest 8000 pg/mL , then adding 100 muL of the 8000 pg/mL standard to both that well and the 4000 pg/mL well, mixing the well contents by rinsing the pipette tip, and adding 100 muL of the 4000 pg/mL standard to the 2000 pg/mL well. Continue these dilutions to the 125 pg/mL standard well, out of which the extra 100 muL should be discarded .