BD OptiBuildTM BV421 Hamster Anti-Mouse CD209b (SIGN-R1)

Description

Multiparameter flow cytometric analysis of CD209b SIGN-R1 expression on BALB/c versus C57BL/6 Mouse Peritoneal Exudate Cells PECs . Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots Mice and were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Hamster Isotype Control Cat. No. 562612, Left Plot or BD OptiBuild BV421 Hamster Anti-Mouse CD209b SIGN-R1 antibody Cat. No. 756157, Right Plot at 0.25 mug/test. A bivariate pseudocolor density plot showing the correlated expression of CD209b SIGN-R1 or Ig Isotype Control Staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD209b SIGN-R1 expression on BALB/c versus C57BL/6 Mouse Peritoneal Exudate Cells PECs . Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots Mice and were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Hamster Isotype Control Cat. No. 562612, Left Plot or BD OptiBuild BV421 Hamster Anti-Mouse CD209b SIGN-R1 antibody Cat. No. 756157, Right Plot at 0.25 mug/test. A bivariate pseudocolor density plot showing the correlated expression of CD209b SIGN-R1 or Ig Isotype Control Staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD209b SIGN-R1 expression on BALB/c versus C57BL/6 Mouse Peritoneal Exudate Cells PECs . Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots Mice and were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Hamster Isotype Control Cat. No. 562612, Left Plot or BD OptiBuild BV421 Hamster Anti-Mouse CD209b SIGN-R1 antibody Cat. No. 756157, Right Plot at 0.25 mug/test. A bivariate pseudocolor density plot showing the correlated expression of CD209b SIGN-R1 or Ig Isotype Control Staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD209b SIGN-R1 expression on BALB/c versus C57BL/6 Mouse Peritoneal Exudate Cells PECs . Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots Mice and were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Hamster Isotype Control Cat. No. 562612, Left Plot or BD OptiBuild BV421 Hamster Anti-Mouse CD209b SIGN-R1 antibody Cat. No. 756157, Right Plot at 0.25 mug/test. A bivariate pseudocolor density plot showing the correlated expression of CD209b SIGN-R1 or Ig Isotype Control Staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD209b SIGN-R1 expression on BALB/c versus C57BL/6 Mouse Peritoneal Exudate Cells PECs . Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots Mice and were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Hamster Isotype Control Cat. No. 562612, Left Plot or BD OptiBuild BV421 Hamster Anti-Mouse CD209b SIGN-R1 antibody Cat. No. 756157, Right Plot at 0.25 mug/test. A bivariate pseudocolor density plot showing the correlated expression of CD209b SIGN-R1 or Ig Isotype Control Staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD209b SIGN-R1 expression on BALB/c versus C57BL/6 Mouse Peritoneal Exudate Cells PECs . Resident peritoneal cells were harvested from BALB/c Top Plots or C57BL/6 Bottom Plots Mice and were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Hamster Isotype Control Cat. No. 562612, Left Plot or BD OptiBuild BV421 Hamster Anti-Mouse CD209b SIGN-R1 antibody Cat. No. 756157, Right Plot at 0.25 mug/test. A bivariate pseudocolor density plot showing the correlated expression of CD209b SIGN-R1 or Ig Isotype Control Staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.