BD OptiBuildTM BV421 Hamster Anti-Mouse FceR1a

Description

Multicolor flow cytometric analysis of FcepsilonRIalpha expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062 , FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088 , and APC Rat Anti-Mouse CD49b Cat. No. 560628 antibodies and with either BD Horizon BV421 Hamster IgG1, kappa Isotype Control Cat. No. 562601; Left Plot or BD OptiBuild BV421 Mouse Anti-FcepsilonRIalpha antibody Cat. No. 750852; Right Plot at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of FcepsilonRIalpha or Ig Isotype control staining versus CD49b was derived from CD3-negative B220-negative gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of FcepsilonRIalpha expression in Mouse MC/9 cells. Cells from the Mouse MC/9 Mast Cells, ATCC CRL-8306 cell line were stained with BD Horizon BV421 Hamster IgG1, kappa Isotype Control Cat. No. 562601; dashed line histogram or BD OptiBuild BV421 Mouse Anti-FcepsilonRIalpha antibody Cat. No. 750852; solid line histogram at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The fluorescence histogram showing FcepsilonRIalpha expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of FcepsilonRIalpha expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062 , FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088 , and APC Rat Anti-Mouse CD49b Cat. No. 560628 antibodies and with either BD Horizon BV421 Hamster IgG1, kappa Isotype Control Cat. No. 562601; Left Plot or BD OptiBuild BV421 Mouse Anti-FcepsilonRIalpha antibody Cat. No. 750852; Right Plot at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of FcepsilonRIalpha or Ig Isotype control staining versus CD49b was derived from CD3-negative B220-negative gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of FcepsilonRIalpha expression in Mouse MC/9 cells. Cells from the Mouse MC/9 Mast Cells, ATCC CRL-8306 cell line were stained with BD Horizon BV421 Hamster IgG1, kappa Isotype Control Cat. No. 562601; dashed line histogram or BD OptiBuild BV421 Mouse Anti-FcepsilonRIalpha antibody Cat. No. 750852; solid line histogram at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The fluorescence histogram showing FcepsilonRIalpha expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of FcepsilonRIalpha expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062 , FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088 , and APC Rat Anti-Mouse CD49b Cat. No. 560628 antibodies and with either BD Horizon BV421 Hamster IgG1, kappa Isotype Control Cat. No. 562601; Left Plot or BD OptiBuild BV421 Mouse Anti-FcepsilonRIalpha antibody Cat. No. 750852; Right Plot at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of FcepsilonRIalpha or Ig Isotype control staining versus CD49b was derived from CD3-negative B220-negative gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of FcepsilonRIalpha expression in Mouse MC/9 cells. Cells from the Mouse MC/9 Mast Cells, ATCC CRL-8306 cell line were stained with BD Horizon BV421 Hamster IgG1, kappa Isotype Control Cat. No. 562601; dashed line histogram or BD OptiBuild BV421 Mouse Anti-FcepsilonRIalpha antibody Cat. No. 750852; solid line histogram at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The fluorescence histogram showing FcepsilonRIalpha expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of FcepsilonRIalpha expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062 , FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088 , and APC Rat Anti-Mouse CD49b Cat. No. 560628 antibodies and with either BD Horizon BV421 Hamster IgG1, kappa Isotype Control Cat. No. 562601; Left Plot or BD OptiBuild BV421 Mouse Anti-FcepsilonRIalpha antibody Cat. No. 750852; Right Plot at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of FcepsilonRIalpha or Ig Isotype control staining versus CD49b was derived from CD3-negative B220-negative gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of FcepsilonRIalpha expression in Mouse MC/9 cells. Cells from the Mouse MC/9 Mast Cells, ATCC CRL-8306 cell line were stained with BD Horizon BV421 Hamster IgG1, kappa Isotype Control Cat. No. 562601; dashed line histogram or BD OptiBuild BV421 Mouse Anti-FcepsilonRIalpha antibody Cat. No. 750852; solid line histogram at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The fluorescence histogram showing FcepsilonRIalpha expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of FcepsilonRIalpha expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062 , FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088 , and APC Rat Anti-Mouse CD49b Cat. No. 560628 antibodies and with either BD Horizon BV421 Hamster IgG1, kappa Isotype Control Cat. No. 562601; Left Plot or BD OptiBuild BV421 Mouse Anti-FcepsilonRIalpha antibody Cat. No. 750852; Right Plot at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of FcepsilonRIalpha or Ig Isotype control staining versus CD49b was derived from CD3-negative B220-negative gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of FcepsilonRIalpha expression in Mouse MC/9 cells. Cells from the Mouse MC/9 Mast Cells, ATCC CRL-8306 cell line were stained with BD Horizon BV421 Hamster IgG1, kappa Isotype Control Cat. No. 562601; dashed line histogram or BD OptiBuild BV421 Mouse Anti-FcepsilonRIalpha antibody Cat. No. 750852; solid line histogram at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The fluorescence histogram showing FcepsilonRIalpha expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of FcepsilonRIalpha expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553062 , FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088 , and APC Rat Anti-Mouse CD49b Cat. No. 560628 antibodies and with either BD Horizon BV421 Hamster IgG1, kappa Isotype Control Cat. No. 562601; Left Plot or BD OptiBuild BV421 Mouse Anti-FcepsilonRIalpha antibody Cat. No. 750852; Right Plot at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of FcepsilonRIalpha or Ig Isotype control staining versus CD49b was derived from CD3-negative B220-negative gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of FcepsilonRIalpha expression in Mouse MC/9 cells. Cells from the Mouse MC/9 Mast Cells, ATCC CRL-8306 cell line were stained with BD Horizon BV421 Hamster IgG1, kappa Isotype Control Cat. No. 562601; dashed line histogram or BD OptiBuild BV421 Mouse Anti-FcepsilonRIalpha antibody Cat. No. 750852; solid line histogram at 0.25 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. The fluorescence histogram showing FcepsilonRIalpha expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.