BD OptiBuildTM BV421 Mouse Anti-C3/C3b/iC3b

Description

Flow cytometric analysis of Human C3/C3b/iC3b deposition on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were treated with either Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336, Left Plot or Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645: Right Plot and were incubated with Human serum as a source of Complement for 30 min at 37C. After washing, the cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dotted line histograms or BD OptiBuild Mouse Anti-C3/C3b/iC3b antibody Cat. No. 752718; solid line histograms at 0.5 mug/test. The fluorescence histograms showing the deposition of human C3/C3b/iC3b were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Human C3/C3b/iC3b deposition on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were treated with either Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336, Left Plot or Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645: Right Plot and were incubated with Human serum as a source of Complement for 30 min at 37C. After washing, the cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dotted line histograms or BD OptiBuild Mouse Anti-C3/C3b/iC3b antibody Cat. No. 752718; solid line histograms at 0.5 mug/test. The fluorescence histograms showing the deposition of human C3/C3b/iC3b were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Mouse C3/C3b/iC3b deposition on C57BL/6 Mouse thymocytes. Thymocytes from a C57BL/6 Mouse were treated with either Purified NA/LE Rat Anti-Mouse CD90.2 antibody Cat. No. 553008, Left Plot or Purified NA/LE Rat IgG2b, kappa Isotype Control Cat. No. 555845, Right Plot and were incubated with Mouse serum as a source of Complement for 20 min at 4C. After washing, the cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dotted line histograms or BD OptiBuild Mouse Anti-C3/C3b/iC3b antibody Cat. No. 752718; solid line histograms at 0.5 mug/test. The fluorescence histograms showing the deposition of Mouse C3/C3b/iC3b were derived from gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Mouse C3/C3b/iC3b deposition on C57BL/6 Mouse thymocytes. Thymocytes from a C57BL/6 Mouse were treated with either Purified NA/LE Rat Anti-Mouse CD90.2 antibody Cat. No. 553008, Left Plot or Purified NA/LE Rat IgG2b, kappa Isotype Control Cat. No. 555845, Right Plot and were incubated with Mouse serum as a source of Complement for 20 min at 4C. After washing, the cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dotted line histograms or BD OptiBuild Mouse Anti-C3/C3b/iC3b antibody Cat. No. 752718; solid line histograms at 0.5 mug/test. The fluorescence histograms showing the deposition of Mouse C3/C3b/iC3b were derived from gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Human C3/C3b/iC3b deposition on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were treated with either Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336, Left Plot or Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645: Right Plot and were incubated with Human serum as a source of Complement for 30 min at 37C. After washing, the cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dotted line histograms or BD OptiBuild Mouse Anti-C3/C3b/iC3b antibody Cat. No. 752718; solid line histograms at 0.5 mug/test. The fluorescence histograms showing the deposition of human C3/C3b/iC3b were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Human C3/C3b/iC3b deposition on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were treated with either Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336, Left Plot or Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645: Right Plot and were incubated with Human serum as a source of Complement for 30 min at 37C. After washing, the cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dotted line histograms or BD OptiBuild Mouse Anti-C3/C3b/iC3b antibody Cat. No. 752718; solid line histograms at 0.5 mug/test. The fluorescence histograms showing the deposition of human C3/C3b/iC3b were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Mouse C3/C3b/iC3b deposition on C57BL/6 Mouse thymocytes. Thymocytes from a C57BL/6 Mouse were treated with either Purified NA/LE Rat Anti-Mouse CD90.2 antibody Cat. No. 553008, Left Plot or Purified NA/LE Rat IgG2b, kappa Isotype Control Cat. No. 555845, Right Plot and were incubated with Mouse serum as a source of Complement for 20 min at 4C. After washing, the cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dotted line histograms or BD OptiBuild Mouse Anti-C3/C3b/iC3b antibody Cat. No. 752718; solid line histograms at 0.5 mug/test. The fluorescence histograms showing the deposition of Mouse C3/C3b/iC3b were derived from gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Mouse C3/C3b/iC3b deposition on C57BL/6 Mouse thymocytes. Thymocytes from a C57BL/6 Mouse were treated with either Purified NA/LE Rat Anti-Mouse CD90.2 antibody Cat. No. 553008, Left Plot or Purified NA/LE Rat IgG2b, kappa Isotype Control Cat. No. 555845, Right Plot and were incubated with Mouse serum as a source of Complement for 20 min at 4C. After washing, the cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dotted line histograms or BD OptiBuild Mouse Anti-C3/C3b/iC3b antibody Cat. No. 752718; solid line histograms at 0.5 mug/test. The fluorescence histograms showing the deposition of Mouse C3/C3b/iC3b were derived from gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Human C3/C3b/iC3b deposition on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were treated with either Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336, Left Plot or Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645: Right Plot and were incubated with Human serum as a source of Complement for 30 min at 37C. After washing, the cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dotted line histograms or BD OptiBuild Mouse Anti-C3/C3b/iC3b antibody Cat. No. 752718; solid line histograms at 0.5 mug/test. The fluorescence histograms showing the deposition of human C3/C3b/iC3b were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Human C3/C3b/iC3b deposition on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were treated with either Purified NA/LE Mouse Anti-Human CD3 antibody Cat. No. 555336, Left Plot or Purified NA/LE Mouse IgG2a, kappa Isotype Control Cat. No. 554645: Right Plot and were incubated with Human serum as a source of Complement for 30 min at 37C. After washing, the cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dotted line histograms or BD OptiBuild Mouse Anti-C3/C3b/iC3b antibody Cat. No. 752718; solid line histograms at 0.5 mug/test. The fluorescence histograms showing the deposition of human C3/C3b/iC3b were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Mouse C3/C3b/iC3b deposition on C57BL/6 Mouse thymocytes. Thymocytes from a C57BL/6 Mouse were treated with either Purified NA/LE Rat Anti-Mouse CD90.2 antibody Cat. No. 553008, Left Plot or Purified NA/LE Rat IgG2b, kappa Isotype Control Cat. No. 555845, Right Plot and were incubated with Mouse serum as a source of Complement for 20 min at 4C. After washing, the cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dotted line histograms or BD OptiBuild Mouse Anti-C3/C3b/iC3b antibody Cat. No. 752718; solid line histograms at 0.5 mug/test. The fluorescence histograms showing the deposition of Mouse C3/C3b/iC3b were derived from gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Mouse C3/C3b/iC3b deposition on C57BL/6 Mouse thymocytes. Thymocytes from a C57BL/6 Mouse were treated with either Purified NA/LE Rat Anti-Mouse CD90.2 antibody Cat. No. 553008, Left Plot or Purified NA/LE Rat IgG2b, kappa Isotype Control Cat. No. 555845, Right Plot and were incubated with Mouse serum as a source of Complement for 20 min at 4C. After washing, the cells were stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dotted line histograms or BD OptiBuild Mouse Anti-C3/C3b/iC3b antibody Cat. No. 752718; solid line histograms at 0.5 mug/test. The fluorescence histograms showing the deposition of Mouse C3/C3b/iC3b were derived from gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.