BD OptiBuildTM BV421 Mouse Anti-Human Carbonic Anhydrase IX

Description

Flow cytometric analysis of Carbonic Anhydrase IX expression on U-87 MG cells. Cells from the Human U-87 MG Glioblastoma, ATCC HTB-14 cell line were stained with BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram or BD OptiBuild BV421 Mouse Anti-Human Carbonic Anhydrase IX antibody Cat. No. 753821; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing Carbonic Anhydrase IX expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Carbonic Anhydrase IX expression on U-87 MG cells. Cells from the Human U-87 MG Glioblastoma, ATCC HTB-14 cell line were stained with BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram or BD OptiBuild BV421 Mouse Anti-Human Carbonic Anhydrase IX antibody Cat. No. 753821; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing Carbonic Anhydrase IX expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Carbonic Anhydrase IX expression on U-87 MG cells. Cells from the Human U-87 MG Glioblastoma, ATCC HTB-14 cell line were stained with BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram or BD OptiBuild BV421 Mouse Anti-Human Carbonic Anhydrase IX antibody Cat. No. 753821; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing Carbonic Anhydrase IX expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Carbonic Anhydrase IX expression on U-87 MG cells. Cells from the Human U-87 MG Glioblastoma, ATCC HTB-14 cell line were stained with BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram or BD OptiBuild BV421 Mouse Anti-Human Carbonic Anhydrase IX antibody Cat. No. 753821; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing Carbonic Anhydrase IX expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Carbonic Anhydrase IX expression on U-87 MG cells. Cells from the Human U-87 MG Glioblastoma, ATCC HTB-14 cell line were stained with BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram or BD OptiBuild BV421 Mouse Anti-Human Carbonic Anhydrase IX antibody Cat. No. 753821; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing Carbonic Anhydrase IX expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Carbonic Anhydrase IX expression on U-87 MG cells. Cells from the Human U-87 MG Glioblastoma, ATCC HTB-14 cell line were stained with BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram or BD OptiBuild BV421 Mouse Anti-Human Carbonic Anhydrase IX antibody Cat. No. 753821; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing Carbonic Anhydrase IX expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.