BD OptiBuildTM BV421 Mouse Anti-Human CD80 (B7-1)

Description

Flow cytometric analysis of CD80 B7-1 expression on Human Raji cells. Cells from the Human Raji Burkitts B cell lymphoma, ATCC CCL-86 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, dashed line histogram or BD OptiBuild BV421 Mouse Anti-Human CD80 B7-1 Cat No. 751723; solid line histogram at 1.0 ug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD80 B7-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Raji cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD80 B7-1 expression on Human Raji cells. Cells from the Human Raji Burkitts B cell lymphoma, ATCC CCL-86 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, dashed line histogram or BD OptiBuild BV421 Mouse Anti-Human CD80 B7-1 Cat No. 751723; solid line histogram at 1.0 ug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD80 B7-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Raji cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD80 B7-1 expression on Human Raji cells. Cells from the Human Raji Burkitts B cell lymphoma, ATCC CCL-86 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, dashed line histogram or BD OptiBuild BV421 Mouse Anti-Human CD80 B7-1 Cat No. 751723; solid line histogram at 1.0 ug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD80 B7-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Raji cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD80 B7-1 expression on Human Raji cells. Cells from the Human Raji Burkitts B cell lymphoma, ATCC CCL-86 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, dashed line histogram or BD OptiBuild BV421 Mouse Anti-Human CD80 B7-1 Cat No. 751723; solid line histogram at 1.0 ug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD80 B7-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Raji cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD80 B7-1 expression on Human Raji cells. Cells from the Human Raji Burkitts B cell lymphoma, ATCC CCL-86 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, dashed line histogram or BD OptiBuild BV421 Mouse Anti-Human CD80 B7-1 Cat No. 751723; solid line histogram at 1.0 ug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD80 B7-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Raji cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD80 B7-1 expression on Human Raji cells. Cells from the Human Raji Burkitts B cell lymphoma, ATCC CCL-86 cell line were preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat No. 562438, dashed line histogram or BD OptiBuild BV421 Mouse Anti-Human CD80 B7-1 Cat No. 751723; solid line histogram at 1.0 ug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD80 B7-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative Raji cells. Flow cytometry and data analysis were performed using a BD FACSCelesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.