BD OptiBuildTM BV421 Mouse Anti-Human IgG2

Description

Flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD OptiBuild BV421 Mouse Anti-Human IgG2 antibody Cat. No. 756047; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD OptiBuild BV421 Mouse Anti-Human IgG2 antibody Cat. No. 756047; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD OptiBuild BV421 Mouse Anti-Human IgG2 antibody Cat. No. 756047; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD OptiBuild BV421 Mouse Anti-Human IgG2 antibody Cat. No. 756047; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD OptiBuild BV421 Mouse Anti-Human IgG2 antibody Cat. No. 756047; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD OptiBuild BV421 Mouse Anti-Human IgG2 antibody Cat. No. 756047; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD OptiBuild BV421 Mouse Anti-Human IgG2 antibody Cat. No. 756047; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD OptiBuild BV421 Mouse Anti-Human IgG2 antibody Cat. No. 756047; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD OptiBuild BV421 Mouse Anti-Human IgG2 antibody Cat. No. 756047; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD OptiBuild BV421 Mouse Anti-Human IgG2 antibody Cat. No. 756047; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD OptiBuild BV421 Mouse Anti-Human IgG2 antibody Cat. No. 756047; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IgG2 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Plot or BD OptiBuild BV421 Mouse Anti-Human IgG2 antibody Cat. No. 756047; Right Plot at 0.03 mug/test. The bivariate pseudocolor density plot showing the correlated expression of cell surface IgG2 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.