BD OptiBuildTM BV421 Mouse Anti-Human Mer

Description

Flow cytometric analysis of Mer expression on human histiocytic lymphoma cells and monocyte-derived macrophages. Left Plot: U-937 cells ATCC CRL-1593.2 were stained with either BD OptiBuild BV421 Mouse Anti-Human Mer Cat. No. 748102, solid line histogram or BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram at 0.5 mug/test. The fluorescence histogram showing Mer or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Middle and Right Plot: Monocytes were isolated from a normal donor and were cultured in complete RPMI medium containing heat-inactivated fetal bovine serum and M-CSF for seven days, with dexamethasone added for the final 3 days for M2 polarization. The macrophages were then stained with PE Mouse Anti-Human CD11b/Mac-1 Cat. No. 555388 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Middle Plot or BD OptiBuild BV421 Mouse Anti-Human Mer Right Plot at 0.5 mug/test. The pseudocolor dot plots showing CD11b staining with or without Mer staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet macrophages. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Flow cytometric analysis of Mer expression on human histiocytic lymphoma cells and monocyte-derived macrophages. Left Plot: U-937 cells ATCC CRL-1593.2 were stained with either BD OptiBuild BV421 Mouse Anti-Human Mer Cat. No. 748102, solid line histogram or BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram at 0.5 mug/test. The fluorescence histogram showing Mer or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Middle and Right Plot: Monocytes were isolated from a normal donor and were cultured in complete RPMI medium containing heat-inactivated fetal bovine serum and M-CSF for seven days, with dexamethasone added for the final 3 days for M2 polarization. The macrophages were then stained with PE Mouse Anti-Human CD11b/Mac-1 Cat. No. 555388 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Middle Plot or BD OptiBuild BV421 Mouse Anti-Human Mer Right Plot at 0.5 mug/test. The pseudocolor dot plots showing CD11b staining with or without Mer staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet macrophages. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Flow cytometric analysis of Mer expression on human histiocytic lymphoma cells and monocyte-derived macrophages. Left Plot: U-937 cells ATCC CRL-1593.2 were stained with either BD OptiBuild BV421 Mouse Anti-Human Mer Cat. No. 748102, solid line histogram or BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram at 0.5 mug/test. The fluorescence histogram showing Mer or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Middle and Right Plot: Monocytes were isolated from a normal donor and were cultured in complete RPMI medium containing heat-inactivated fetal bovine serum and M-CSF for seven days, with dexamethasone added for the final 3 days for M2 polarization. The macrophages were then stained with PE Mouse Anti-Human CD11b/Mac-1 Cat. No. 555388 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Middle Plot or BD OptiBuild BV421 Mouse Anti-Human Mer Right Plot at 0.5 mug/test. The pseudocolor dot plots showing CD11b staining with or without Mer staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet macrophages. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Show More Flow cytometric analysis of Mer expression on human histiocytic lymphoma cells and monocyte-derived macrophages. Left Plot: U-937 cells ATCC CRL-1593.2 were stained with either BD OptiBuild BV421 Mouse Anti-Human Mer Cat. No. 748102, solid line histogram or BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram at 0.5 mug/test. The fluorescence histogram showing Mer or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Middle and Right Plot: Monocytes were isolated from a normal donor and were cultured in complete RPMI medium containing heat-inactivated fetal bovine serum and M-CSF for seven days, with dexamethasone added for the final 3 days for M2 polarization. The macrophages were then stained with PE Mouse Anti-Human CD11b/Mac-1 Cat. No. 555388 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Middle Plot or BD OptiBuild BV421 Mouse Anti-Human Mer Right Plot at 0.5 mug/test. The pseudocolor dot plots showing CD11b staining with or without Mer staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet macrophages. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Show More Flow cytometric analysis of Mer expression on human histiocytic lymphoma cells and monocyte-derived macrophages. Left Plot: U-937 cells ATCC CRL-1593.2 were stained with either BD OptiBuild BV421 Mouse Anti-Human Mer Cat. No. 748102, solid line histogram or BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram at 0.5 mug/test. The fluorescence histogram showing Mer or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Middle and Right Plot: Monocytes were isolated from a normal donor and were cultured in complete RPMI medium containing heat-inactivated fetal bovine serum and M-CSF for seven days, with dexamethasone added for the final 3 days for M2 polarization. The macrophages were then stained with PE Mouse Anti-Human CD11b/Mac-1 Cat. No. 555388 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Middle Plot or BD OptiBuild BV421 Mouse Anti-Human Mer Right Plot at 0.5 mug/test. The pseudocolor dot plots showing CD11b staining with or without Mer staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet macrophages. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Flow cytometric analysis of Mer expression on human histiocytic lymphoma cells and monocyte-derived macrophages. Left Plot: U-937 cells ATCC CRL-1593.2 were stained with either BD OptiBuild BV421 Mouse Anti-Human Mer Cat. No. 748102, solid line histogram or BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histogram at 0.5 mug/test. The fluorescence histogram showing Mer or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Middle and Right Plot: Monocytes were isolated from a normal donor and were cultured in complete RPMI medium containing heat-inactivated fetal bovine serum and M-CSF for seven days, with dexamethasone added for the final 3 days for M2 polarization. The macrophages were then stained with PE Mouse Anti-Human CD11b/Mac-1 Cat. No. 555388 and either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Middle Plot or BD OptiBuild BV421 Mouse Anti-Human Mer Right Plot at 0.5 mug/test. The pseudocolor dot plots showing CD11b staining with or without Mer staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet macrophages. Flow cytometry and data analysis were performed using a BD LSRFortessa X20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot.