BD OptiBuildTM BV421 Mouse Anti-Mouse EVA1

Description

Flow cytometric analysis of EVA1 expression on Mouse thymocytes. C57BL/6 mouse thymocytes were stained with Alexa Fluor 647 Rat Anti-Mouse CD4 Cat. No. 557681 , BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786/565968 , FITC Rat Anti-Mouse CD44 Cat. No. 553133/561859 , BD Horizon BV650 Rat Anti-Mouse CD25 Cat. No. 564021 , and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD OptiBuild BV421 Mouse Anti-Mouse EVA1 antibody Cat. No. 752399 at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Isotype control Dotted line histogram or EVA1 Solid line histogram staining on DN3 cells CD4 - CD8a - CD44 - CD25 + are shown and compared to EVA1 Dashed line histogram staining on DN1 cells CD4 - CD8a - CD44 + CD25 - . The fluorescence histograms showing the expression of EVA1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative thymocytes. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of EVA1 expression on Mouse thymocytes. C57BL/6 mouse thymocytes were stained with Alexa Fluor 647 Rat Anti-Mouse CD4 Cat. No. 557681 , BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786/565968 , FITC Rat Anti-Mouse CD44 Cat. No. 553133/561859 , BD Horizon BV650 Rat Anti-Mouse CD25 Cat. No. 564021 , and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD OptiBuild BV421 Mouse Anti-Mouse EVA1 antibody Cat. No. 752399 at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Isotype control Dotted line histogram or EVA1 Solid line histogram staining on DN3 cells CD4 - CD8a - CD44 - CD25 + are shown and compared to EVA1 Dashed line histogram staining on DN1 cells CD4 - CD8a - CD44 + CD25 - . The fluorescence histograms showing the expression of EVA1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative thymocytes. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of EVA1 expression on Mouse thymocytes. C57BL/6 mouse thymocytes were stained with Alexa Fluor 647 Rat Anti-Mouse CD4 Cat. No. 557681 , BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786/565968 , FITC Rat Anti-Mouse CD44 Cat. No. 553133/561859 , BD Horizon BV650 Rat Anti-Mouse CD25 Cat. No. 564021 , and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD OptiBuild BV421 Mouse Anti-Mouse EVA1 antibody Cat. No. 752399 at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Isotype control Dotted line histogram or EVA1 Solid line histogram staining on DN3 cells CD4 - CD8a - CD44 - CD25 + are shown and compared to EVA1 Dashed line histogram staining on DN1 cells CD4 - CD8a - CD44 + CD25 - . The fluorescence histograms showing the expression of EVA1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative thymocytes. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of EVA1 expression on Mouse thymocytes. C57BL/6 mouse thymocytes were stained with Alexa Fluor 647 Rat Anti-Mouse CD4 Cat. No. 557681 , BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786/565968 , FITC Rat Anti-Mouse CD44 Cat. No. 553133/561859 , BD Horizon BV650 Rat Anti-Mouse CD25 Cat. No. 564021 , and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD OptiBuild BV421 Mouse Anti-Mouse EVA1 antibody Cat. No. 752399 at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Isotype control Dotted line histogram or EVA1 Solid line histogram staining on DN3 cells CD4 - CD8a - CD44 - CD25 + are shown and compared to EVA1 Dashed line histogram staining on DN1 cells CD4 - CD8a - CD44 + CD25 - . The fluorescence histograms showing the expression of EVA1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative thymocytes. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of EVA1 expression on Mouse thymocytes. C57BL/6 mouse thymocytes were stained with Alexa Fluor 647 Rat Anti-Mouse CD4 Cat. No. 557681 , BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786/565968 , FITC Rat Anti-Mouse CD44 Cat. No. 553133/561859 , BD Horizon BV650 Rat Anti-Mouse CD25 Cat. No. 564021 , and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD OptiBuild BV421 Mouse Anti-Mouse EVA1 antibody Cat. No. 752399 at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Isotype control Dotted line histogram or EVA1 Solid line histogram staining on DN3 cells CD4 - CD8a - CD44 - CD25 + are shown and compared to EVA1 Dashed line histogram staining on DN1 cells CD4 - CD8a - CD44 + CD25 - . The fluorescence histograms showing the expression of EVA1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative thymocytes. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of EVA1 expression on Mouse thymocytes. C57BL/6 mouse thymocytes were stained with Alexa Fluor 647 Rat Anti-Mouse CD4 Cat. No. 557681 , BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786/565968 , FITC Rat Anti-Mouse CD44 Cat. No. 553133/561859 , BD Horizon BV650 Rat Anti-Mouse CD25 Cat. No. 564021 , and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438 or BD OptiBuild BV421 Mouse Anti-Mouse EVA1 antibody Cat. No. 752399 at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Isotype control Dotted line histogram or EVA1 Solid line histogram staining on DN3 cells CD4 - CD8a - CD44 - CD25 + are shown and compared to EVA1 Dashed line histogram staining on DN1 cells CD4 - CD8a - CD44 + CD25 - . The fluorescence histograms showing the expression of EVA1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative thymocytes. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.