BD OptiBuildTM BV421 Rat Anti-Mouse Axl

Description

Flow cytometric analysis of Axl expression on mouse cell lines. Left Plot: NIH3T3 ATCC CRL-1658 mouse embryonic fibroblasts were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD OptiBuild BV421 Rat Anti-Mouse Axl Cat. No. 748028; solid line histogram at 1.0 mug/test. Right Plot: ST-2 DSMZ ACC 333 mouse bone marrow stromal cells were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD OptiBuild BV421 Rat Anti-Mouse Axl Cat. No. 748028; solid line histogram at 1.0 mug/test. Cell Dissociation Buffer Gibco 13151014 was used to detach the cell lines before staining. The fluorescence histograms showing Axl or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Flow cytometric analysis of Axl expression on mouse cell lines. Left Plot: NIH3T3 ATCC CRL-1658 mouse embryonic fibroblasts were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD OptiBuild BV421 Rat Anti-Mouse Axl Cat. No. 748028; solid line histogram at 1.0 mug/test. Right Plot: ST-2 DSMZ ACC 333 mouse bone marrow stromal cells were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD OptiBuild BV421 Rat Anti-Mouse Axl Cat. No. 748028; solid line histogram at 1.0 mug/test. Cell Dissociation Buffer Gibco 13151014 was used to detach the cell lines before staining. The fluorescence histograms showing Axl or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Flow cytometric analysis of Axl expression on mouse cell lines. Left Plot: NIH3T3 ATCC CRL-1658 mouse embryonic fibroblasts were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD OptiBuild BV421 Rat Anti-Mouse Axl Cat. No. 748028; solid line histogram at 1.0 mug/test. Right Plot: ST-2 DSMZ ACC 333 mouse bone marrow stromal cells were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD OptiBuild BV421 Rat Anti-Mouse Axl Cat. No. 748028; solid line histogram at 1.0 mug/test. Cell Dissociation Buffer Gibco 13151014 was used to detach the cell lines before staining. The fluorescence histograms showing Axl or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Show More Flow cytometric analysis of Axl expression on mouse cell lines. Left Plot: NIH3T3 ATCC CRL-1658 mouse embryonic fibroblasts were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD OptiBuild BV421 Rat Anti-Mouse Axl Cat. No. 748028; solid line histogram at 1.0 mug/test. Right Plot: ST-2 DSMZ ACC 333 mouse bone marrow stromal cells were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD OptiBuild BV421 Rat Anti-Mouse Axl Cat. No. 748028; solid line histogram at 1.0 mug/test. Cell Dissociation Buffer Gibco 13151014 was used to detach the cell lines before staining. The fluorescence histograms showing Axl or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Show More Flow cytometric analysis of Axl expression on mouse cell lines. Left Plot: NIH3T3 ATCC CRL-1658 mouse embryonic fibroblasts were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD OptiBuild BV421 Rat Anti-Mouse Axl Cat. No. 748028; solid line histogram at 1.0 mug/test. Right Plot: ST-2 DSMZ ACC 333 mouse bone marrow stromal cells were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD OptiBuild BV421 Rat Anti-Mouse Axl Cat. No. 748028; solid line histogram at 1.0 mug/test. Cell Dissociation Buffer Gibco 13151014 was used to detach the cell lines before staining. The fluorescence histograms showing Axl or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Flow cytometric analysis of Axl expression on mouse cell lines. Left Plot: NIH3T3 ATCC CRL-1658 mouse embryonic fibroblasts were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD OptiBuild BV421 Rat Anti-Mouse Axl Cat. No. 748028; solid line histogram at 1.0 mug/test. Right Plot: ST-2 DSMZ ACC 333 mouse bone marrow stromal cells were stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histogram or BD OptiBuild BV421 Rat Anti-Mouse Axl Cat. No. 748028; solid line histogram at 1.0 mug/test. Cell Dissociation Buffer Gibco 13151014 was used to detach the cell lines before staining. The fluorescence histograms showing Axl or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot.