BD OptiBuildTM BV421 Rat Anti-Mouse CD192 (CCR2)

Description

Two-color flow cytometric analysis of CD192 CCR2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C Cat No. 553129/561083 , and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse CD192 CCR2 Cat. No. 747963; Right Plot at 0.06 mug/test. The pseudocolor dot plots showing the correlated expression of CD192 CCR2 or Ig Isotype control staining versus LY-6G and Ly-6C were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Two-color flow cytometric analysis of CD192 CCR2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C Cat No. 553129/561083 , and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse CD192 CCR2 Cat. No. 747963; Right Plot at 0.06 mug/test. The pseudocolor dot plots showing the correlated expression of CD192 CCR2 or Ig Isotype control staining versus LY-6G and Ly-6C were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Two-color flow cytometric analysis of CD192 CCR2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C Cat No. 553129/561083 , and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse CD192 CCR2 Cat. No. 747963; Right Plot at 0.06 mug/test. The pseudocolor dot plots showing the correlated expression of CD192 CCR2 or Ig Isotype control staining versus LY-6G and Ly-6C were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Show More Two-color flow cytometric analysis of CD192 CCR2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C Cat No. 553129/561083 , and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse CD192 CCR2 Cat. No. 747963; Right Plot at 0.06 mug/test. The pseudocolor dot plots showing the correlated expression of CD192 CCR2 or Ig Isotype control staining versus LY-6G and Ly-6C were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Show More Two-color flow cytometric analysis of CD192 CCR2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C Cat No. 553129/561083 , and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse CD192 CCR2 Cat. No. 747963; Right Plot at 0.06 mug/test. The pseudocolor dot plots showing the correlated expression of CD192 CCR2 or Ig Isotype control staining versus LY-6G and Ly-6C were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. Two-color flow cytometric analysis of CD192 CCR2 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with APC Rat Anti-Mouse Ly-6G and Ly-6C Cat No. 553129/561083 , and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse CD192 CCR2 Cat. No. 747963; Right Plot at 0.06 mug/test. The pseudocolor dot plots showing the correlated expression of CD192 CCR2 or Ig Isotype control staining versus LY-6G and Ly-6C were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot.