BD OptiBuildTM BV421 Rat Anti-Mouse CD369 (Clec7a)

Description

Flow cytometric analysis of CD369 Clec7a expression on mouse peripheral blood leucocytes PBL and peritoneal exudate cells PEC . PBL: BALB/c mouse PBL were stained with FITC Rat Anti-Mouse Ly-6G antibody Cat. No. 551460 or 561105 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Plot A or BD OptiBuild BV421 Rat Anti-Mouse CD369 Clec7a antibody Cat. No. 749795; Plot B at 1 mug/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 Clec7a or Ig Isotype control staining versus Ly-6G were derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. PEC: BALB/c mouse thioglycolate-elicited PEC were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565854 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Plot C or BD OptiBuild BV421 Rat Anti-Mouse CD369 Clec7a antibody Cat. No. 749795; PlotD at 1 mug/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 Clec7a or Ig Isotype control versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable PEC. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are qualification data only and do not represent a specific OptiBuild lot. Flow cytometric analysis of CD369 Clec7a expression on mouse peripheral blood leucocytes PBL and peritoneal exudate cells PEC . PBL: BALB/c mouse PBL were stained with FITC Rat Anti-Mouse Ly-6G antibody Cat. No. 551460 or 561105 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Plot A or BD OptiBuild BV421 Rat Anti-Mouse CD369 Clec7a antibody Cat. No. 749795; Plot B at 1 mug/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 Clec7a or Ig Isotype control staining versus Ly-6G were derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. PEC: BALB/c mouse thioglycolate-elicited PEC were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565854 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Plot C or BD OptiBuild BV421 Rat Anti-Mouse CD369 Clec7a antibody Cat. No. 749795; PlotD at 1 mug/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 Clec7a or Ig Isotype control versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable PEC. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are qualification data only and do not represent a specific OptiBuild lot. Flow cytometric analysis of CD369 Clec7a expression on mouse peripheral blood leucocytes PBL and peritoneal exudate cells PEC . PBL: BALB/c mouse PBL were stained with FITC Rat Anti-Mouse Ly-6G antibody Cat. No. 551460 or 561105 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Plot A or BD OptiBuild BV421 Rat Anti-Mouse CD369 Clec7a antibody Cat. No. 749795; Plot B at 1 mug/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 Clec7a or Ig Isotype control staining versus Ly-6G were derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. PEC: BALB/c mouse thioglycolate-elicited PEC were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565854 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Plot C or BD OptiBuild BV421 Rat Anti-Mouse CD369 Clec7a antibody Cat. No. 749795; PlotD at 1 mug/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 Clec7a or Ig Isotype control versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable PEC. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are qualification data only and do not represent a specific OptiBuild lot. Show More Flow cytometric analysis of CD369 Clec7a expression on mouse peripheral blood leucocytes PBL and peritoneal exudate cells PEC . PBL: BALB/c mouse PBL were stained with FITC Rat Anti-Mouse Ly-6G antibody Cat. No. 551460 or 561105 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Plot A or BD OptiBuild BV421 Rat Anti-Mouse CD369 Clec7a antibody Cat. No. 749795; Plot B at 1 mug/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 Clec7a or Ig Isotype control staining versus Ly-6G were derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. PEC: BALB/c mouse thioglycolate-elicited PEC were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565854 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Plot C or BD OptiBuild BV421 Rat Anti-Mouse CD369 Clec7a antibody Cat. No. 749795; PlotD at 1 mug/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 Clec7a or Ig Isotype control versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable PEC. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are qualification data only and do not represent a specific OptiBuild lot. Show More Flow cytometric analysis of CD369 Clec7a expression on mouse peripheral blood leucocytes PBL and peritoneal exudate cells PEC . PBL: BALB/c mouse PBL were stained with FITC Rat Anti-Mouse Ly-6G antibody Cat. No. 551460 or 561105 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Plot A or BD OptiBuild BV421 Rat Anti-Mouse CD369 Clec7a antibody Cat. No. 749795; Plot B at 1 mug/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 Clec7a or Ig Isotype control staining versus Ly-6G were derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. PEC: BALB/c mouse thioglycolate-elicited PEC were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565854 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Plot C or BD OptiBuild BV421 Rat Anti-Mouse CD369 Clec7a antibody Cat. No. 749795; PlotD at 1 mug/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 Clec7a or Ig Isotype control versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable PEC. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are qualification data only and do not represent a specific OptiBuild lot. Flow cytometric analysis of CD369 Clec7a expression on mouse peripheral blood leucocytes PBL and peritoneal exudate cells PEC . PBL: BALB/c mouse PBL were stained with FITC Rat Anti-Mouse Ly-6G antibody Cat. No. 551460 or 561105 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Plot A or BD OptiBuild BV421 Rat Anti-Mouse CD369 Clec7a antibody Cat. No. 749795; Plot B at 1 mug/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 Clec7a or Ig Isotype control staining versus Ly-6G were derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. PEC: BALB/c mouse thioglycolate-elicited PEC were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 antibody Cat. No. 565854 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Plot C or BD OptiBuild BV421 Rat Anti-Mouse CD369 Clec7a antibody Cat. No. 749795; PlotD at 1 mug/test. Two-parameter pseudocolor density plots showing the correlated expression of CD369 Clec7a or Ig Isotype control versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable PEC. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown in this Technical Data Sheet are qualification data only and do not represent a specific OptiBuild lot.