BD OptiBuildTM BV421 Rat Anti-Mouse DCIR4 (Clec4a1)

Description

Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on Mouse splenic leucocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 751751; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on Mouse splenic leucocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 751751; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on Mouse splenic leucocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 751751; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on Mouse splenic leucocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 751751; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on Mouse splenic leucocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 751751; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on Mouse splenic leucocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 751751; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on Mouse splenic leucocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 751751; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on Mouse splenic leucocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 751751; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on Mouse splenic leucocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 751751; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on Mouse splenic leucocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 751751; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on Mouse splenic leucocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 751751; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of DCIR4 Clec4a1 expression on Mouse splenic leucocytes. C57BL/6 mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-CD11b antibody Cat. No. 557686 and with either BD Horizon BV421 Rat IgG1, kappa Isotype Control Cat. No. 562868; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse DCIR4 Clec4a1 antibody Cat. No. 751751; Right Plot at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of DCIR4 Clec4a1 or Ig Isotype control staining versus CD11b was derived from gated events with the forward and side-light scatter characteristics of viable 7-AAD-negative leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.