BD OptiBuildTM BV421 Rat Anti-Mouse Mer

Description

Two-color flow cytometric analysis of Mer expression on normal mouse peritoneal cavity cells PEC . PEC from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 Cat. No. 565853 or 565854 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse Mer Cat. No. 747837; Right Plot at 1 mug/test. The pseudocolor dot plots showing Mer or Ig Isotype Control staining versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable peritoneal cavity cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. . Two-color flow cytometric analysis of Mer expression on normal mouse peritoneal cavity cells PEC . PEC from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 Cat. No. 565853 or 565854 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse Mer Cat. No. 747837; Right Plot at 1 mug/test. The pseudocolor dot plots showing Mer or Ig Isotype Control staining versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable peritoneal cavity cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. . Two-color flow cytometric analysis of Mer expression on normal mouse peritoneal cavity cells PEC . PEC from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 Cat. No. 565853 or 565854 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse Mer Cat. No. 747837; Right Plot at 1 mug/test. The pseudocolor dot plots showing Mer or Ig Isotype Control staining versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable peritoneal cavity cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. . Show More Two-color flow cytometric analysis of Mer expression on normal mouse peritoneal cavity cells PEC . PEC from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 Cat. No. 565853 or 565854 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse Mer Cat. No. 747837; Right Plot at 1 mug/test. The pseudocolor dot plots showing Mer or Ig Isotype Control staining versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable peritoneal cavity cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. . Show More Two-color flow cytometric analysis of Mer expression on normal mouse peritoneal cavity cells PEC . PEC from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 Cat. No. 565853 or 565854 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse Mer Cat. No. 747837; Right Plot at 1 mug/test. The pseudocolor dot plots showing Mer or Ig Isotype Control staining versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable peritoneal cavity cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. . Two-color flow cytometric analysis of Mer expression on normal mouse peritoneal cavity cells PEC . PEC from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 or 553142 . The cells were then stained with Alexa Fluor 647 Rat Anti-Mouse F4/80 Cat. No. 565853 or 565854 and either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; Left Plot or BD OptiBuild BV421 Rat Anti-Mouse Mer Cat. No. 747837; Right Plot at 1 mug/test. The pseudocolor dot plots showing Mer or Ig Isotype Control staining versus F4/80 were derived from gated events with the forward and side light-scatter characteristics of viable peritoneal cavity cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. The above is qualification data only and does not represent a specific OptiBuild lot. .