BD PharmingenTM Alexa Fluor 488 Mouse anti-Actin

Description

Immunofluorescent staining of human cell lines. U-2 OS cells ATCC HTB-96 were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor 488 Mouse anti-Actin pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 855 Bioimager System with a 20x objective and merged using BD Attovision software. This antibody also stains A549 ATCC CCL-185 and HeLa ATCC CCL-2 cells. Triton X-100 is not recommended as a permeabilization agent with this antibody. Immunofluorescent staining of human cell lines. U-2 OS cells ATCC HTB-96 were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor 488 Mouse anti-Actin pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 855 Bioimager System with a 20x objective and merged using BD Attovision software. This antibody also stains A549 ATCC CCL-185 and HeLa ATCC CCL-2 cells. Triton X-100 is not recommended as a permeabilization agent with this antibody. Show More Immunofluorescent staining of human cell lines. U-2 OS cells ATCC HTB-96 were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor 488 Mouse anti-Actin pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 855 Bioimager System with a 20x objective and merged using BD Attovision software. This antibody also stains A549 ATCC CCL-185 and HeLa ATCC CCL-2 cells. Triton X-100 is not recommended as a permeabilization agent with this antibody.