BD PharmingenTM Alexa Fluor 488 Mouse anti-beta-Catenin

Description

Flow cytometric analysis of beta-Catenin expression by mouse bone marrow stromal cells. M2-10B4 cells ATCC CRL-1972 were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were stained with either Alexa Fluor 488 Mouse IgG1 kappa Isotype Control 557721; open histogram or Alexa Fluor 488 Mouse anti-beta-Catenin Cat. No. 562505; filled histogram . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Immunofluorescent staining and imaging of human breast adenocarcinoma cells. Human MCF-7 cells ATCC HTB-22 were processed and stained according to the Recommended Assay Procedure for Bioimaging cultured cells. The cells were stained with Alexa Fluor 488 Mouse anti-beta-Catenin monoclonal antibody Cat. No. 562505, pseudo-colored green and counter-stained with Hoechst 33342 Solution Cat. No. 561908; pseudo-colored blue . The images were captured using a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Flow cytometric analysis of beta-Catenin expression by mouse bone marrow stromal cells. M2-10B4 cells ATCC CRL-1972 were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were stained with either Alexa Fluor 488 Mouse IgG1 kappa Isotype Control 557721; open histogram or Alexa Fluor 488 Mouse anti-beta-Catenin Cat. No. 562505; filled histogram . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Immunofluorescent staining and imaging of human breast adenocarcinoma cells. Human MCF-7 cells ATCC HTB-22 were processed and stained according to the Recommended Assay Procedure for Bioimaging cultured cells. The cells were stained with Alexa Fluor 488 Mouse anti-beta-Catenin monoclonal antibody Cat. No. 562505, pseudo-colored green and counter-stained with Hoechst 33342 Solution Cat. No. 561908; pseudo-colored blue . The images were captured using a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Show More Flow cytometric analysis of beta-Catenin expression by mouse bone marrow stromal cells. M2-10B4 cells ATCC CRL-1972 were fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were stained with either Alexa Fluor 488 Mouse IgG1 kappa Isotype Control 557721; open histogram or Alexa Fluor 488 Mouse anti-beta-Catenin Cat. No. 562505; filled histogram . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Immunofluorescent staining and imaging of human breast adenocarcinoma cells. Human MCF-7 cells ATCC HTB-22 were processed and stained according to the Recommended Assay Procedure for Bioimaging cultured cells. The cells were stained with Alexa Fluor 488 Mouse anti-beta-Catenin monoclonal antibody Cat. No. 562505, pseudo-colored green and counter-stained with Hoechst 33342 Solution Cat. No. 561908; pseudo-colored blue . The images were captured using a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software.