BD PharmingenTM Alexa Fluor 488 Mouse anti-Cytochrome c

Description

Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were seeded in a 96-well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with Triton X-100, stained with Alexa Fluor 488 Mouse anti-Cytochrome c pseudo colored green , and the nuclei were counterstained with Hoechst 33342 pseudo colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 Cell Analyzer with a 20x objective and merged using BD Attovision software. This antibody also stains A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells using Triton X-100, cold methanol, or Saponin for permeabilization see Recommended Assay Procedure . Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were seeded in a 96-well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with Triton X-100, stained with Alexa Fluor 488 Mouse anti-Cytochrome c pseudo colored green , and the nuclei were counterstained with Hoechst 33342 pseudo colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 Cell Analyzer with a 20x objective and merged using BD Attovision software. This antibody also stains A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells using Triton X-100, cold methanol, or Saponin for permeabilization see Recommended Assay Procedure . Show More Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were seeded in a 96-well imaging plate Cat. No. 353219 at 10,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with Triton X-100, stained with Alexa Fluor 488 Mouse anti-Cytochrome c pseudo colored green , and the nuclei were counterstained with Hoechst 33342 pseudo colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 Cell Analyzer with a 20x objective and merged using BD Attovision software. This antibody also stains A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells using Triton X-100, cold methanol, or Saponin for permeabilization see Recommended Assay Procedure .