BD PharmingenTM Alexa Fluor 488 Mouse anti-E-Cadherin

Description

Immunofluorescent staining of mouse small intestine left panel and human breast adenocarcinoma middle panel . Mouse small intestine and MCF-7 cells ATCC HTB-22 were processed and stained according to the Recommended Assay Procedures for tissue sections and cultured cells, respectively. Both specimens were stained with Alexa Fluor 488 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green and counter-stained with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Panel: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Immunofluorescent staining of mouse small intestine left panel and human breast adenocarcinoma middle panel . Mouse small intestine and MCF-7 cells ATCC HTB-22 were processed and stained according to the Recommended Assay Procedures for tissue sections and cultured cells, respectively. Both specimens were stained with Alexa Fluor 488 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green and counter-stained with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Panel: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Immunofluorescent staining of mouse small intestine left panel and human breast adenocarcinoma middle panel . Mouse small intestine and MCF-7 cells ATCC HTB-22 were processed and stained according to the Recommended Assay Procedures for tissue sections and cultured cells, respectively. Both specimens were stained with Alexa Fluor 488 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green and counter-stained with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Panel: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Immunofluorescent staining of mouse small intestine left panel and human breast adenocarcinoma middle panel . Mouse small intestine and MCF-7 cells ATCC HTB-22 were processed and stained according to the Recommended Assay Procedures for tissue sections and cultured cells, respectively. Both specimens were stained with Alexa Fluor 488 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green and counter-stained with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Panel: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Show More Immunofluorescent staining of mouse small intestine left panel and human breast adenocarcinoma middle panel . Mouse small intestine and MCF-7 cells ATCC HTB-22 were processed and stained according to the Recommended Assay Procedures for tissue sections and cultured cells, respectively. Both specimens were stained with Alexa Fluor 488 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green and counter-stained with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Panel: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Show More Immunofluorescent staining of mouse small intestine left panel and human breast adenocarcinoma middle panel . Mouse small intestine and MCF-7 cells ATCC HTB-22 were processed and stained according to the Recommended Assay Procedures for tissue sections and cultured cells, respectively. Both specimens were stained with Alexa Fluor 488 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green and counter-stained with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Panel: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Show More Immunofluorescent staining of mouse small intestine left panel and human breast adenocarcinoma middle panel . Mouse small intestine and MCF-7 cells ATCC HTB-22 were processed and stained according to the Recommended Assay Procedures for tissue sections and cultured cells, respectively. Both specimens were stained with Alexa Fluor 488 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green and counter-stained with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Panel: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Immunofluorescent staining of mouse small intestine left panel and human breast adenocarcinoma middle panel . Mouse small intestine and MCF-7 cells ATCC HTB-22 were processed and stained according to the Recommended Assay Procedures for tissue sections and cultured cells, respectively. Both specimens were stained with Alexa Fluor 488 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green and counter-stained with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Panel: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Immunofluorescent staining of mouse small intestine left panel and human breast adenocarcinoma middle panel . Mouse small intestine and MCF-7 cells ATCC HTB-22 were processed and stained according to the Recommended Assay Procedures for tissue sections and cultured cells, respectively. Both specimens were stained with Alexa Fluor 488 Mouse anti-E-Cadherin monoclonal antibody pseudo-colored green and counter-stained with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. Right Panel: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 . E-Cadherin is observable at 120kDa. Left Panel: A431 lysate ATCC CRL-1555; human epithelial carcinoma was blotted at 1:10000 & 1:20000 Lanes 1 & 2 respectively; 30 second exposure . Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 Lanes 1 & 2 respectively; 30 second exposure . Middle Right Panel: 293F cells transfected with human E-Cadherin CDH1 was blotted at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure . Right Panel: 293 cells transfected with human P-Cadherin CDH3 was blotted using Purified Mouse Anti-E-Cadherin Cat. No. 610181/610182 at 1:2500 & 1: 5000 Lanes 1 & 2 respectively; 5 second exposure .