BD PharmingenTM Alexa Fluor 488 Mouse anti-EZH2

Description

LEFT: Flow cytometric analysis of EZH2 expression in a human cell line. Jurkat cells ATCC, TIB-152 were harvested, fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 and stained with matching concentrations of either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 557721; dashed line histogram or Alexa Fluor 488 Mouse anti-EZH2 antibody Cat. No. 562479; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable Jurkat cells. Flow cytometry was performed using a BD FacsCanto II Flow Cytometer System. RIGHT: Immunoflourescent staining and image analysis of EZH2 expression in a human cell line. HeLa cells ATCC, CCL-2 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton-X 100 Sigma Cat. No. X-100 , and stained with Alexa Fluor 488 Mouse anti-EZH2 monoclonal antibody Cat. No. 562479; pseudo colored green at 10 mug/mL. BD Pharmingen Hoechst 33342 solution Cat. No. 561908; pseudo-colored blue was used for counterstaining. The image was captured using a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. LEFT: Flow cytometric analysis of EZH2 expression in a human cell line. Jurkat cells ATCC, TIB-152 were harvested, fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 and stained with matching concentrations of either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 557721; dashed line histogram or Alexa Fluor 488 Mouse anti-EZH2 antibody Cat. No. 562479; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable Jurkat cells. Flow cytometry was performed using a BD FacsCanto II Flow Cytometer System. RIGHT: Immunoflourescent staining and image analysis of EZH2 expression in a human cell line. HeLa cells ATCC, CCL-2 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton-X 100 Sigma Cat. No. X-100 , and stained with Alexa Fluor 488 Mouse anti-EZH2 monoclonal antibody Cat. No. 562479; pseudo colored green at 10 mug/mL. BD Pharmingen Hoechst 33342 solution Cat. No. 561908; pseudo-colored blue was used for counterstaining. The image was captured using a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. LEFT: Flow cytometric analysis of EZH2 expression in a human cell line. Jurkat cells ATCC, TIB-152 were harvested, fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 and stained with matching concentrations of either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 557721; dashed line histogram or Alexa Fluor 488 Mouse anti-EZH2 antibody Cat. No. 562479; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable Jurkat cells. Flow cytometry was performed using a BD FacsCanto II Flow Cytometer System. RIGHT: Immunoflourescent staining and image analysis of EZH2 expression in a human cell line. HeLa cells ATCC, CCL-2 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton-X 100 Sigma Cat. No. X-100 , and stained with Alexa Fluor 488 Mouse anti-EZH2 monoclonal antibody Cat. No. 562479; pseudo colored green at 10 mug/mL. BD Pharmingen Hoechst 33342 solution Cat. No. 561908; pseudo-colored blue was used for counterstaining. The image was captured using a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Show More LEFT: Flow cytometric analysis of EZH2 expression in a human cell line. Jurkat cells ATCC, TIB-152 were harvested, fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 and stained with matching concentrations of either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 557721; dashed line histogram or Alexa Fluor 488 Mouse anti-EZH2 antibody Cat. No. 562479; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable Jurkat cells. Flow cytometry was performed using a BD FacsCanto II Flow Cytometer System. RIGHT: Immunoflourescent staining and image analysis of EZH2 expression in a human cell line. HeLa cells ATCC, CCL-2 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton-X 100 Sigma Cat. No. X-100 , and stained with Alexa Fluor 488 Mouse anti-EZH2 monoclonal antibody Cat. No. 562479; pseudo colored green at 10 mug/mL. BD Pharmingen Hoechst 33342 solution Cat. No. 561908; pseudo-colored blue was used for counterstaining. The image was captured using a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Show More LEFT: Flow cytometric analysis of EZH2 expression in a human cell line. Jurkat cells ATCC, TIB-152 were harvested, fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 and stained with matching concentrations of either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 557721; dashed line histogram or Alexa Fluor 488 Mouse anti-EZH2 antibody Cat. No. 562479; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable Jurkat cells. Flow cytometry was performed using a BD FacsCanto II Flow Cytometer System. RIGHT: Immunoflourescent staining and image analysis of EZH2 expression in a human cell line. HeLa cells ATCC, CCL-2 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton-X 100 Sigma Cat. No. X-100 , and stained with Alexa Fluor 488 Mouse anti-EZH2 monoclonal antibody Cat. No. 562479; pseudo colored green at 10 mug/mL. BD Pharmingen Hoechst 33342 solution Cat. No. 561908; pseudo-colored blue was used for counterstaining. The image was captured using a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. LEFT: Flow cytometric analysis of EZH2 expression in a human cell line. Jurkat cells ATCC, TIB-152 were harvested, fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 and stained with matching concentrations of either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 557721; dashed line histogram or Alexa Fluor 488 Mouse anti-EZH2 antibody Cat. No. 562479; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable Jurkat cells. Flow cytometry was performed using a BD FacsCanto II Flow Cytometer System. RIGHT: Immunoflourescent staining and image analysis of EZH2 expression in a human cell line. HeLa cells ATCC, CCL-2 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton-X 100 Sigma Cat. No. X-100 , and stained with Alexa Fluor 488 Mouse anti-EZH2 monoclonal antibody Cat. No. 562479; pseudo colored green at 10 mug/mL. BD Pharmingen Hoechst 33342 solution Cat. No. 561908; pseudo-colored blue was used for counterstaining. The image was captured using a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software.