BD PharmingenTM Alexa Fluor 488 Mouse anti-GATA3

Description

Immunofluorescent staining of human breast adenocarcinoma. MCF-7 cells ATCC HTB-22 were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor 488 Mouse anti-GATA3 monoclonal antibody pseudo-colored red, which appears pink when co-localized with the blue , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. We have observed somewhat dimmer staining when using Triton X-100 for permeabilization see Recommended Assay Procedure . Immunofluorescent staining of human breast adenocarcinoma. MCF-7 cells ATCC HTB-22 were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor 488 Mouse anti-GATA3 monoclonal antibody pseudo-colored red, which appears pink when co-localized with the blue , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. We have observed somewhat dimmer staining when using Triton X-100 for permeabilization see Recommended Assay Procedure . Show More Immunofluorescent staining of human breast adenocarcinoma. MCF-7 cells ATCC HTB-22 were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor 488 Mouse anti-GATA3 monoclonal antibody pseudo-colored red, which appears pink when co-localized with the blue , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 Bioimager System with a 20x objective and merged using BD Attovision software. We have observed somewhat dimmer staining when using Triton X-100 for permeabilization see Recommended Assay Procedure .