BD PharmingenTM Alexa Fluor 488 Mouse anti-GM130

Description

Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were cultured, fixed, permeabilized with Triton X-100, stained with Alexa Fluor 488 Mouse anti-GM130 pseudo colored green , and counter stained with Hoechst 33342 pseudo colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 Cell Analyzer with a 20x objective and merged using BD AttoVision software. This antibody also stains A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells, and it also worked with the Saponin and cold methanol fix/perm protocols see Recommended Assay Procedure . Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were cultured, fixed, permeabilized with Triton X-100, stained with Alexa Fluor 488 Mouse anti-GM130 pseudo colored green , and counter stained with Hoechst 33342 pseudo colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 Cell Analyzer with a 20x objective and merged using BD AttoVision software. This antibody also stains A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells, and it also worked with the Saponin and cold methanol fix/perm protocols see Recommended Assay Procedure . Show More Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were cultured, fixed, permeabilized with Triton X-100, stained with Alexa Fluor 488 Mouse anti-GM130 pseudo colored green , and counter stained with Hoechst 33342 pseudo colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 Cell Analyzer with a 20x objective and merged using BD AttoVision software. This antibody also stains A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells, and it also worked with the Saponin and cold methanol fix/perm protocols see Recommended Assay Procedure .