BD PharmingenTM Alexa Fluor 488 Mouse Anti-Human BMI-1

Description

Image analysis of BMI-1 expressed in human osteosarcoma cell line. U-2 OS cells ATCC; HTB-96 were fixed with BD Cytofix Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100 Sigma, Cat. No. X-100 , and stained with Alexa Fluor 488 Mouse Anti-Human-BMI-1 antibody 5 mug/ml; pseudo-colored green . Counter-staining was with DAPI pseudo-colored blue . The image was captured using a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Flow cytometric analysis of BMI-1 expression in a human osteosarcoma cell line . U-2 OS cells ATCC; HTB-96 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control dashed line histogram, Cat. No. 557782 or Alexa Fluor 488 Mouse anti-Human BMI-1 antibody solid line histogram, Cat. No. 562636 at matched concentrations. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD LSR II Flow Cytometry System. Image analysis of BMI-1 expressed in human osteosarcoma cell line. U-2 OS cells ATCC; HTB-96 were fixed with BD Cytofix Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100 Sigma, Cat. No. X-100 , and stained with Alexa Fluor 488 Mouse Anti-Human-BMI-1 antibody 5 mug/ml; pseudo-colored green . Counter-staining was with DAPI pseudo-colored blue . The image was captured using a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Show More Flow cytometric analysis of BMI-1 expression in a human osteosarcoma cell line . U-2 OS cells ATCC; HTB-96 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control dashed line histogram, Cat. No. 557782 or Alexa Fluor 488 Mouse anti-Human BMI-1 antibody solid line histogram, Cat. No. 562636 at matched concentrations. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD LSR II Flow Cytometry System. Show More Image analysis of BMI-1 expressed in human osteosarcoma cell line. U-2 OS cells ATCC; HTB-96 were fixed with BD Cytofix Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton X-100 Sigma, Cat. No. X-100 , and stained with Alexa Fluor 488 Mouse Anti-Human-BMI-1 antibody 5 mug/ml; pseudo-colored green . Counter-staining was with DAPI pseudo-colored blue . The image was captured using a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Flow cytometric analysis of BMI-1 expression in a human osteosarcoma cell line . U-2 OS cells ATCC; HTB-96 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control dashed line histogram, Cat. No. 557782 or Alexa Fluor 488 Mouse anti-Human BMI-1 antibody solid line histogram, Cat. No. 562636 at matched concentrations. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD LSR II Flow Cytometry System.