
567006 · 100 Tests
BDB Bioscience · Cat: 567006
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD107a expression in Jurkat cells. Jurkat cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 The cells were subsequently stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histogram or Alexa Fluor 488 Mouse anti-Human CD107a antibody Cat. No. 567006/567007; solid line histogram in BD Perm/Wash Buffer. The fluorescence histogram showing CD107a expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD107a expression in Jurkat cells. Jurkat cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 The cells were subsequently stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histogram or Alexa Fluor 488 Mouse anti-Human CD107a antibody Cat. No. 567006/567007; solid line histogram in BD Perm/Wash Buffer. The fluorescence histogram showing CD107a expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD107a expression in Jurkat cells. Jurkat cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 The cells were subsequently stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histogram or Alexa Fluor 488 Mouse anti-Human CD107a antibody Cat. No. 567006/567007; solid line histogram in BD Perm/Wash Buffer. The fluorescence histogram showing CD107a expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.