BD PharmingenTM Alexa Fluor 488 Mouse Anti-Human CD19

Description

Immunohistofluorescent analysis of CD19 expression by cells within human tonsil left panel . A human tonsil cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Pharmingen Purified Mouse Anti-Human CD11c antibody Cat. No. 565805 followed by BD Horizon BV480 Goat Anti-Mouse Ig second step antibody Cat. No. 564877, pseudo-colored green . Sections were thoroughly washed, then stained with Alexa Fluor 488 Mouse Anti-Human CD19 antibody Cat. No. 557697, pseudo-colored blue and BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Profile of peripheral blood lymphocytes analyzed by flow cytometry right panel . Immunohistofluorescent analysis of CD19 expression by cells within human tonsil left panel . A human tonsil cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Pharmingen Purified Mouse Anti-Human CD11c antibody Cat. No. 565805 followed by BD Horizon BV480 Goat Anti-Mouse Ig second step antibody Cat. No. 564877, pseudo-colored green . Sections were thoroughly washed, then stained with Alexa Fluor 488 Mouse Anti-Human CD19 antibody Cat. No. 557697, pseudo-colored blue and BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Profile of peripheral blood lymphocytes analyzed by flow cytometry right panel . Show More Immunohistofluorescent analysis of CD19 expression by cells within human tonsil left panel . A human tonsil cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with BD Pharmingen Purified Mouse Anti-Human CD11c antibody Cat. No. 565805 followed by BD Horizon BV480 Goat Anti-Mouse Ig second step antibody Cat. No. 564877, pseudo-colored green . Sections were thoroughly washed, then stained with Alexa Fluor 488 Mouse Anti-Human CD19 antibody Cat. No. 557697, pseudo-colored blue and BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Profile of peripheral blood lymphocytes analyzed by flow cytometry right panel .