BD PharmingenTM Alexa Fluor 488 Mouse Anti-Human CD324 (E-Cadherin)

Description

Flow cytometric analysis of CD324 E-cadherin expression in human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI were harvested with Cell Dissociation Buffer Life Technologies . Please note that the epitope is sensitive to Accutase detachment. The cells were stained with either Alexa Fluor 488 Mouse anti-Human CD324 antibody Cat. No. 563570, solid line or Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 557782, dashed line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of H9 human ES cells. Flow cytometry was performed on a BD LSRFortessa Flow Cytometry System. Immunofluorescent Staining of CD324 E-Cadherin on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown in mTESR1 media Stem Cell Technologies were fixed with BD Cytofix fixation buffer Cat. No. 554655 . Cells were stained with Alexa Fluor 488 Mouse Anti-Human CD324 E-Cadherin monoclonal antibody Cat. No. 563570, pseudo-colored green at 5 mug/mL. Counter-staining was with DAPI pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software. Flow cytometric analysis of CD324 E-cadherin expression in human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI were harvested with Cell Dissociation Buffer Life Technologies . Please note that the epitope is sensitive to Accutase detachment. The cells were stained with either Alexa Fluor 488 Mouse anti-Human CD324 antibody Cat. No. 563570, solid line or Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 557782, dashed line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of H9 human ES cells. Flow cytometry was performed on a BD LSRFortessa Flow Cytometry System. Show More Immunofluorescent Staining of CD324 E-Cadherin on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown in mTESR1 media Stem Cell Technologies were fixed with BD Cytofix fixation buffer Cat. No. 554655 . Cells were stained with Alexa Fluor 488 Mouse Anti-Human CD324 E-Cadherin monoclonal antibody Cat. No. 563570, pseudo-colored green at 5 mug/mL. Counter-staining was with DAPI pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software. Show More Flow cytometric analysis of CD324 E-cadherin expression in human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI were harvested with Cell Dissociation Buffer Life Technologies . Please note that the epitope is sensitive to Accutase detachment. The cells were stained with either Alexa Fluor 488 Mouse anti-Human CD324 antibody Cat. No. 563570, solid line or Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 557782, dashed line at matched concentrations. Histograms were derived from gated events based on light scattering characteristics of H9 human ES cells. Flow cytometry was performed on a BD LSRFortessa Flow Cytometry System. Immunofluorescent Staining of CD324 E-Cadherin on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown in mTESR1 media Stem Cell Technologies were fixed with BD Cytofix fixation buffer Cat. No. 554655 . Cells were stained with Alexa Fluor 488 Mouse Anti-Human CD324 E-Cadherin monoclonal antibody Cat. No. 563570, pseudo-colored green at 5 mug/mL. Counter-staining was with DAPI pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD AttoVision Software.