BD PharmingenTM Alexa Fluor 488 Mouse anti-Human FoxP3

Description

Flow cytometric analysis of Alexa 488 anti-human FoxP3 on resting PBMC. Human PBMC were stained with APC anti-human CD4 clone RPA-T4, Cat. No. 555349 and PE anti-human CD25 Clone M-A251, Cat. No. 555432 simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with Alexa 488 anti-Human FoxP3 clone 259D/C7; Cat No. 560047 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of Alexa 488 anti-human FoxP3 on resting PBMC. Human PBMC were stained with APC anti-human CD4 clone RPA-T4, Cat. No. 555349 and PE anti-human CD25 Clone M-A251, Cat. No. 555432 simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with Alexa 488 anti-Human FoxP3 clone 259D/C7; Cat No. 560047 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of Alexa 488 anti-human FoxP3 on resting PBMC. Human PBMC were stained with APC anti-human CD4 clone RPA-T4, Cat. No. 555349 and PE anti-human CD25 Clone M-A251, Cat. No. 555432 simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with Alexa 488 anti-Human FoxP3 clone 259D/C7; Cat No. 560047 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System. Show More Flow cytometric analysis of Alexa 488 anti-human FoxP3 on resting PBMC. Human PBMC were stained with APC anti-human CD4 clone RPA-T4, Cat. No. 555349 and PE anti-human CD25 Clone M-A251, Cat. No. 555432 simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with Alexa 488 anti-Human FoxP3 clone 259D/C7; Cat No. 560047 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System. Show More Flow cytometric analysis of Alexa 488 anti-human FoxP3 on resting PBMC. Human PBMC were stained with APC anti-human CD4 clone RPA-T4, Cat. No. 555349 and PE anti-human CD25 Clone M-A251, Cat. No. 555432 simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with Alexa 488 anti-Human FoxP3 clone 259D/C7; Cat No. 560047 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of Alexa 488 anti-human FoxP3 on resting PBMC. Human PBMC were stained with APC anti-human CD4 clone RPA-T4, Cat. No. 555349 and PE anti-human CD25 Clone M-A251, Cat. No. 555432 simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with Alexa 488 anti-Human FoxP3 clone 259D/C7; Cat No. 560047 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System.