BD PharmingenTM Alexa Fluor 488 Mouse anti-Human FoxP3

Description

Flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with PerCP-Cy5.5 anti-human CD4 clone L200, Cat. No. 552838 and PE anti-human CD25 Clone 2A3, Cat. No. 341009 simultaneously. The PBMC were fixed and permeabilized see Recommended Assay Procedure followed by intracellular staining with Alexa Fluor 488 Mouse anti-Human FoxP3 clone 236A/E7; Cat No. 561181 . Two-color flow cytometric dot plots were derived from gated events based on the light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+, respectively, shown as either FoxP3 versus CD25 left panel or CD4 versus FoxP3 right panel . Flow cytometry was performed on a BD LSRII Flow Cytometer System. Flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with PerCP-Cy5.5 anti-human CD4 clone L200, Cat. No. 552838 and PE anti-human CD25 Clone 2A3, Cat. No. 341009 simultaneously. The PBMC were fixed and permeabilized see Recommended Assay Procedure followed by intracellular staining with Alexa Fluor 488 Mouse anti-Human FoxP3 clone 236A/E7; Cat No. 561181 . Two-color flow cytometric dot plots were derived from gated events based on the light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+, respectively, shown as either FoxP3 versus CD25 left panel or CD4 versus FoxP3 right panel . Flow cytometry was performed on a BD LSRII Flow Cytometer System. Flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with PerCP-Cy5.5 anti-human CD4 clone L200, Cat. No. 552838 and PE anti-human CD25 Clone 2A3, Cat. No. 341009 simultaneously. The PBMC were fixed and permeabilized see Recommended Assay Procedure followed by intracellular staining with Alexa Fluor 488 Mouse anti-Human FoxP3 clone 236A/E7; Cat No. 561181 . Two-color flow cytometric dot plots were derived from gated events based on the light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+, respectively, shown as either FoxP3 versus CD25 left panel or CD4 versus FoxP3 right panel . Flow cytometry was performed on a BD LSRII Flow Cytometer System. Show More Flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with PerCP-Cy5.5 anti-human CD4 clone L200, Cat. No. 552838 and PE anti-human CD25 Clone 2A3, Cat. No. 341009 simultaneously. The PBMC were fixed and permeabilized see Recommended Assay Procedure followed by intracellular staining with Alexa Fluor 488 Mouse anti-Human FoxP3 clone 236A/E7; Cat No. 561181 . Two-color flow cytometric dot plots were derived from gated events based on the light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+, respectively, shown as either FoxP3 versus CD25 left panel or CD4 versus FoxP3 right panel . Flow cytometry was performed on a BD LSRII Flow Cytometer System. Show More Flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with PerCP-Cy5.5 anti-human CD4 clone L200, Cat. No. 552838 and PE anti-human CD25 Clone 2A3, Cat. No. 341009 simultaneously. The PBMC were fixed and permeabilized see Recommended Assay Procedure followed by intracellular staining with Alexa Fluor 488 Mouse anti-Human FoxP3 clone 236A/E7; Cat No. 561181 . Two-color flow cytometric dot plots were derived from gated events based on the light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+, respectively, shown as either FoxP3 versus CD25 left panel or CD4 versus FoxP3 right panel . Flow cytometry was performed on a BD LSRII Flow Cytometer System. Flow cytometric analysis of FoxP3 expressed in human lymphocytes. Human peripheral blood mononuclear cells PBMC were stained with PerCP-Cy5.5 anti-human CD4 clone L200, Cat. No. 552838 and PE anti-human CD25 Clone 2A3, Cat. No. 341009 simultaneously. The PBMC were fixed and permeabilized see Recommended Assay Procedure followed by intracellular staining with Alexa Fluor 488 Mouse anti-Human FoxP3 clone 236A/E7; Cat No. 561181 . Two-color flow cytometric dot plots were derived from gated events based on the light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+, respectively, shown as either FoxP3 versus CD25 left panel or CD4 versus FoxP3 right panel . Flow cytometry was performed on a BD LSRII Flow Cytometer System.