BD PharmingenTM Alexa Fluor 488 Mouse anti-Human IL-17A

Description

Flow cytometric analysis of Alexa Fluor 488 anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with Alexa Fluor 488 anti-human IL-17A Cat. No. 560488/560489 and PE anti-human IFN-gamma Cat. No. 559327; left panel and APC anti-human IL-4 Cat. No. 554486; right panel . The dot plots were derived from a lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System . Flow cytometric analysis of Alexa Fluor 488 anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with Alexa Fluor 488 anti-human IL-17A Cat. No. 560488/560489 and PE anti-human IFN-gamma Cat. No. 559327; left panel and APC anti-human IL-4 Cat. No. 554486; right panel . The dot plots were derived from a lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System . Flow cytometric analysis of Alexa Fluor 488 anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with Alexa Fluor 488 anti-human IL-17A Cat. No. 560488/560489 and PE anti-human IFN-gamma Cat. No. 559327; left panel and APC anti-human IL-4 Cat. No. 554486; right panel . The dot plots were derived from a lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System . Show More Flow cytometric analysis of Alexa Fluor 488 anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with Alexa Fluor 488 anti-human IL-17A Cat. No. 560488/560489 and PE anti-human IFN-gamma Cat. No. 559327; left panel and APC anti-human IL-4 Cat. No. 554486; right panel . The dot plots were derived from a lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System . Show More Flow cytometric analysis of Alexa Fluor 488 anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with Alexa Fluor 488 anti-human IL-17A Cat. No. 560488/560489 and PE anti-human IFN-gamma Cat. No. 559327; left panel and APC anti-human IL-4 Cat. No. 554486; right panel . The dot plots were derived from a lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System . Flow cytometric analysis of Alexa Fluor 488 anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with Alexa Fluor 488 anti-human IL-17A Cat. No. 560488/560489 and PE anti-human IFN-gamma Cat. No. 559327; left panel and APC anti-human IL-4 Cat. No. 554486; right panel . The dot plots were derived from a lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System .