BD PharmingenTM Alexa Fluor 488 Mouse anti-Human Nanog

Description

Alexa Fluor 488 anti-human Nanog staining of human embryonic stem cells. H9 cells WiCell, Madison, WI , passage 34, were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with Alexa Fluor 488 anti-human Nanog Cat. No. 560791, solid line or Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No.557702, dashed line . Flow cytometry was performed on a BD LSR II flow cytometry system. This reagent will also work in BD Phosflow Perm II and III buffers Cat. No. 558052,558050 respectively . Immunoflourescent staining of human embryonic stem cells with Alexa Fluor 488 anti-human Nanog. H9 cells WiCell, Madison, WI , passage 45, grown in mTESR1 media StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were fixed in BD Cytofix buffer, permeabilized, and stained with Alexa Fluor 488 anti-Human Nanog monoclonal antibody pseudo-colored green at 2.5 mug/mL. Cell nuclei were counter-stained with Hoechst 33342 Cat. No. 561908, pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Permeabilization was done using BD Phosflow Perm/Wash buffer I for this antibody; Triton X-100 is also suitable for permeabilization. Alexa Fluor 488 anti-human Nanog staining of human embryonic stem cells. H9 cells WiCell, Madison, WI , passage 34, were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with Alexa Fluor 488 anti-human Nanog Cat. No. 560791, solid line or Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No.557702, dashed line . Flow cytometry was performed on a BD LSR II flow cytometry system. This reagent will also work in BD Phosflow Perm II and III buffers Cat. No. 558052,558050 respectively . Show More Immunoflourescent staining of human embryonic stem cells with Alexa Fluor 488 anti-human Nanog. H9 cells WiCell, Madison, WI , passage 45, grown in mTESR1 media StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were fixed in BD Cytofix buffer, permeabilized, and stained with Alexa Fluor 488 anti-Human Nanog monoclonal antibody pseudo-colored green at 2.5 mug/mL. Cell nuclei were counter-stained with Hoechst 33342 Cat. No. 561908, pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Permeabilization was done using BD Phosflow Perm/Wash buffer I for this antibody; Triton X-100 is also suitable for permeabilization. Show More Alexa Fluor 488 anti-human Nanog staining of human embryonic stem cells. H9 cells WiCell, Madison, WI , passage 34, were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with Alexa Fluor 488 anti-human Nanog Cat. No. 560791, solid line or Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No.557702, dashed line . Flow cytometry was performed on a BD LSR II flow cytometry system. This reagent will also work in BD Phosflow Perm II and III buffers Cat. No. 558052,558050 respectively . Immunoflourescent staining of human embryonic stem cells with Alexa Fluor 488 anti-human Nanog. H9 cells WiCell, Madison, WI , passage 45, grown in mTESR1 media StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were fixed in BD Cytofix buffer, permeabilized, and stained with Alexa Fluor 488 anti-Human Nanog monoclonal antibody pseudo-colored green at 2.5 mug/mL. Cell nuclei were counter-stained with Hoechst 33342 Cat. No. 561908, pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Permeabilization was done using BD Phosflow Perm/Wash buffer I for this antibody; Triton X-100 is also suitable for permeabilization.