BD PharmingenTM Alexa Fluor 488 Mouse anti-Human Sox17

Description

Flow cytometric analysis of Sox17 in definitive endoderm derived from human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days DAmour et al, 2005 in RPMI medium supplemented with 0.5 % FBS, 1 L-glutamine, and 100 ng/ml Activin A R&D Systems . Control ES cells left panel and day-3 differentiated cells right panel were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa isotype control dashed lines, Cat. No.557782 or Alexa Fluor 488 Mouse Anti-Human Sox17 antibody solid lines at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of the human ES and H9-derived endoderm cells, respectively. Flow cytometry was performed on a BD LSRFortessa flow cytometry system. Immunofluorescent staining of Sox17 on definitive endoderm derived from human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 33 grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days in RPMI supplemented with 0.5 % FBS, 1 L-glutamine, and 100 ng/ml Activin A R&D Systems DAmour et al, 2005 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton-X 100, and stained with Alexa Fluor 488 Mouse anti-Human Sox17 monoclonal antibody pseudo-colored green . Counter-staining was with Hoechst 33342 pseudo-colored blue . The image was captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software . Flow cytometric analysis of Sox17 in definitive endoderm derived from human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days DAmour et al, 2005 in RPMI medium supplemented with 0.5 % FBS, 1 L-glutamine, and 100 ng/ml Activin A R&D Systems . Control ES cells left panel and day-3 differentiated cells right panel were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa isotype control dashed lines, Cat. No.557782 or Alexa Fluor 488 Mouse Anti-Human Sox17 antibody solid lines at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of the human ES and H9-derived endoderm cells, respectively. Flow cytometry was performed on a BD LSRFortessa flow cytometry system. Show More Immunofluorescent staining of Sox17 on definitive endoderm derived from human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 33 grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days in RPMI supplemented with 0.5 % FBS, 1 L-glutamine, and 100 ng/ml Activin A R&D Systems DAmour et al, 2005 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton-X 100, and stained with Alexa Fluor 488 Mouse anti-Human Sox17 monoclonal antibody pseudo-colored green . Counter-staining was with Hoechst 33342 pseudo-colored blue . The image was captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software . Show More Flow cytometric analysis of Sox17 in definitive endoderm derived from human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days DAmour et al, 2005 in RPMI medium supplemented with 0.5 % FBS, 1 L-glutamine, and 100 ng/ml Activin A R&D Systems . Control ES cells left panel and day-3 differentiated cells right panel were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa isotype control dashed lines, Cat. No.557782 or Alexa Fluor 488 Mouse Anti-Human Sox17 antibody solid lines at matched concentrations. The histograms were derived from gated events based on light scattering characteristics of the human ES and H9-derived endoderm cells, respectively. Flow cytometry was performed on a BD LSRFortessa flow cytometry system. Immunofluorescent staining of Sox17 on definitive endoderm derived from human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 33 grown on an irradiated mouse embryonic feeder layer were differentiated to definitive endoderm for 3 days in RPMI supplemented with 0.5 % FBS, 1 L-glutamine, and 100 ng/ml Activin A R&D Systems DAmour et al, 2005 . The cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with 0.1 % Triton-X 100, and stained with Alexa Fluor 488 Mouse anti-Human Sox17 monoclonal antibody pseudo-colored green . Counter-staining was with Hoechst 33342 pseudo-colored blue . The image was captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software .