BD PharmingenTM Alexa Fluor 488 Mouse Anti-Human TGF-beta1

Description

Flow cytometric analysis of human TGF-beta1 expressed by TGF-beta1-transfected P3UI cells. Untransfected mouse P3UI myeloma cells dashed line histogram and human TGF-beta1-transfected P3UI cells solid line histogram were fixed and permeabilized for 30 minutes with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 and washed with BD Perm/Wash Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with Alexa Fluor 488 Mouse Anti-Human TGF-beta1 antibody Cat. No. 562545 . The flow cytometric fluorescence histograms were derived from gated events with the forward- and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of human TGF-beta1 expressed by TGF-beta1-transfected P3UI cells. Untransfected mouse P3UI myeloma cells dashed line histogram and human TGF-beta1-transfected P3UI cells solid line histogram were fixed and permeabilized for 30 minutes with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 and washed with BD Perm/Wash Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with Alexa Fluor 488 Mouse Anti-Human TGF-beta1 antibody Cat. No. 562545 . The flow cytometric fluorescence histograms were derived from gated events with the forward- and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of human TGF-beta1 expressed by TGF-beta1-transfected P3UI cells. Untransfected mouse P3UI myeloma cells dashed line histogram and human TGF-beta1-transfected P3UI cells solid line histogram were fixed and permeabilized for 30 minutes with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 and washed with BD Perm/Wash Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with Alexa Fluor 488 Mouse Anti-Human TGF-beta1 antibody Cat. No. 562545 . The flow cytometric fluorescence histograms were derived from gated events with the forward- and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System.