BD PharmingenTM Alexa Fluor 488 Mouse anti-Ki-67

Description

Flow cytometric analysis of Ki-67 expression by proliferating Jurkat and noncycling human peripheral blood mononuclear cells PBMC . Jurkat and PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with Alexa Fluor 488 Mouse Anti-Ki-67 antibody Cat. No. 561165 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then RNase A Sigma Cat. No. R-5500 treated and counterstained with Propidium Iodide Staining Solution Cat. No. 556463 to stain DNA . Two-color flow cytometric dot plots showing the correlated expression patterns of Propidium Iodide DNA staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II flow cytometry system. Flow cytometric analysis of Ki-67 expression by proliferating Jurkat and noncycling human peripheral blood mononuclear cells PBMC . Jurkat and PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with Alexa Fluor 488 Mouse Anti-Ki-67 antibody Cat. No. 561165 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then RNase A Sigma Cat. No. R-5500 treated and counterstained with Propidium Iodide Staining Solution Cat. No. 556463 to stain DNA . Two-color flow cytometric dot plots showing the correlated expression patterns of Propidium Iodide DNA staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II flow cytometry system. Flow cytometric analysis of Ki-67 expression by proliferating Jurkat and noncycling human peripheral blood mononuclear cells PBMC . Jurkat and PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with Alexa Fluor 488 Mouse Anti-Ki-67 antibody Cat. No. 561165 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then RNase A Sigma Cat. No. R-5500 treated and counterstained with Propidium Iodide Staining Solution Cat. No. 556463 to stain DNA . Two-color flow cytometric dot plots showing the correlated expression patterns of Propidium Iodide DNA staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II flow cytometry system. Show More Flow cytometric analysis of Ki-67 expression by proliferating Jurkat and noncycling human peripheral blood mononuclear cells PBMC . Jurkat and PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with Alexa Fluor 488 Mouse Anti-Ki-67 antibody Cat. No. 561165 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then RNase A Sigma Cat. No. R-5500 treated and counterstained with Propidium Iodide Staining Solution Cat. No. 556463 to stain DNA . Two-color flow cytometric dot plots showing the correlated expression patterns of Propidium Iodide DNA staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II flow cytometry system. Show More Flow cytometric analysis of Ki-67 expression by proliferating Jurkat and noncycling human peripheral blood mononuclear cells PBMC . Jurkat and PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with Alexa Fluor 488 Mouse Anti-Ki-67 antibody Cat. No. 561165 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then RNase A Sigma Cat. No. R-5500 treated and counterstained with Propidium Iodide Staining Solution Cat. No. 556463 to stain DNA . Two-color flow cytometric dot plots showing the correlated expression patterns of Propidium Iodide DNA staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II flow cytometry system. Flow cytometric analysis of Ki-67 expression by proliferating Jurkat and noncycling human peripheral blood mononuclear cells PBMC . Jurkat and PBMC were fixed and permeabilized with 70 % ice cold ethanol, washed, and stained with Alexa Fluor 488 Mouse Anti-Ki-67 antibody Cat. No. 561165 according to the BD Biosciences support protocol, Flow Cytometry Staining Protocol for Detection of Ki-67. The cells were then RNase A Sigma Cat. No. R-5500 treated and counterstained with Propidium Iodide Staining Solution Cat. No. 556463 to stain DNA . Two-color flow cytometric dot plots showing the correlated expression patterns of Propidium Iodide DNA staining versus Ki-67 were derived from gated events with the forward and side light-scatter characteristics of intact Jurkat cells Left Panel or PBMC Right Panel . Flow cytometry was performed using a BD LSR II flow cytometry system.