BD PharmingenTM Alexa Fluor 488 Mouse anti-Oct3/4

Description

Flow cytometric analysis of Alexa Fluor 488 anti Oct3/4 in H9 cells. H9 human embryonic stem ES cells WiCell, Madison, WI were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37C, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with either Alexa Fluor 488 Mouse anti-Oct3/4 Cat. No. 560253; solid line histogram or Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572, dashed line histogram . Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Flow cytometric analysis of Alexa Fluor 488 anti Oct3/4 in H9 cells. H9 human embryonic stem ES cells WiCell, Madison, WI were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37C, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with either Alexa Fluor 488 Mouse anti-Oct3/4 Cat. No. 560253; solid line histogram or Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572, dashed line histogram . Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Show More Flow cytometric analysis of Alexa Fluor 488 anti Oct3/4 in H9 cells. H9 human embryonic stem ES cells WiCell, Madison, WI were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37C, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with either Alexa Fluor 488 Mouse anti-Oct3/4 Cat. No. 560253; solid line histogram or Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572, dashed line histogram . Flow cytometry was performed on a BD FACSCanto II flow cytometry system.