
562274 ·
BDB Bioscience · Cat: 562274
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Description
Flow cytometric analysis of PDX-1 in mouse pancreatic tumor insulinoma cells. Beta-TC-6 ATCC CRL-11506 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, isotype control Cat. No. 557721, dashed line or Alexa Fluor 488 Mouse anti-PDX-1 monoclonal antibody Cat.. No. 562274, solid line at matched concentrations. Flow cytometry was performed on a BD LSRFortessa flow cytometry system. This antibody has been found to be reactive with both mouse and human PDX-1, and it can also be used on fixed cells that were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . Flow cytometric analysis of PDX-1 in mouse pancreatic tumor insulinoma cells. Beta-TC-6 ATCC CRL-11506 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, isotype control Cat. No. 557721, dashed line or Alexa Fluor 488 Mouse anti-PDX-1 monoclonal antibody Cat.. No. 562274, solid line at matched concentrations. Flow cytometry was performed on a BD LSRFortessa flow cytometry system. This antibody has been found to be reactive with both mouse and human PDX-1, and it can also be used on fixed cells that were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 . Show More Flow cytometric analysis of PDX-1 in mouse pancreatic tumor insulinoma cells. Beta-TC-6 ATCC CRL-11506 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, isotype control Cat. No. 557721, dashed line or Alexa Fluor 488 Mouse anti-PDX-1 monoclonal antibody Cat.. No. 562274, solid line at matched concentrations. Flow cytometry was performed on a BD LSRFortessa flow cytometry system. This antibody has been found to be reactive with both mouse and human PDX-1, and it can also be used on fixed cells that were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 .