BD PharmingenTM Alexa Fluor 488 Mouse anti-Sox2

Description

Flow cytometric analysis of Sox2 expression on H9 cells. H9 human embryonic stem ES cells WiCell, Madison, WI were fixed with Fixation Buffer Cat. No. 554655 for 10 minutes at 37C, permeabilized with Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with either Alexa Fluor 488 Mouse anti-Sox2 Cat. No. 560301; solid line or Alexa Fluor 488 Mouse IgG2a Isotype Control Cat. No.558055; dashed line . The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable H9 stem cells. Flow cytometry was performed on a BD FACSCanto II. Flow cytometric analysis of Sox2 expression on H9 cells. H9 human embryonic stem ES cells WiCell, Madison, WI were fixed with Fixation Buffer Cat. No. 554655 for 10 minutes at 37C, permeabilized with Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with either Alexa Fluor 488 Mouse anti-Sox2 Cat. No. 560301; solid line or Alexa Fluor 488 Mouse IgG2a Isotype Control Cat. No.558055; dashed line . The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable H9 stem cells. Flow cytometry was performed on a BD FACSCanto II. Show More Flow cytometric analysis of Sox2 expression on H9 cells. H9 human embryonic stem ES cells WiCell, Madison, WI were fixed with Fixation Buffer Cat. No. 554655 for 10 minutes at 37C, permeabilized with Perm Buffer III Cat. No. 558050 on ice for 30 minutes, and then stained with either Alexa Fluor 488 Mouse anti-Sox2 Cat. No. 560301; solid line or Alexa Fluor 488 Mouse IgG2a Isotype Control Cat. No.558055; dashed line . The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable H9 stem cells. Flow cytometry was performed on a BD FACSCanto II.