BD PharmingenTM Alexa Fluor 488 Mouse anti-Sox2

Description

Analysis of Sox2 on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 and stained with matching concentrations of either Alexa Fluor 488 Mouse IgG1, kappa isotype control dashed line, Cat. No. 557721 or Alexa Fluor 488 Mouse anti-Sox2 monoclonal antibody solid line . Histograms were derived from gated events based on light scattering characteristics of the H9 cell line. Flow cytometry was performed on a BD LSR II flow cytometry system. Analysis of Sox2 on human ES cell-derived neural stem cells NSC . NSC derived from H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 , and stained with matching concentrations of either Alexa Fluor 488 Mouse IgG1, kappa isotype control dashed line, Cat. No. 557721 or Alexa Fluor 488 Mouse Anti-Sox2 monoclonal antibody solid line . Histograms were derived from gated events based on light scattering characteristics of the H9-derived NSC. Flow cytometry was performed on a BD LSR II flow cytometry system. Immunoflourescent staining of human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI , passage 33 grown on irradiated mouse embryonic fibroblasts, were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat No. 558050 , and stained with Alexa Fluor 488 Mouse anti-Sox2 monoclonal antibody pseudo colored green at 1.2 mug/mL. Counter-staining of cell nuclei was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Analysis of Sox2 on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 and stained with matching concentrations of either Alexa Fluor 488 Mouse IgG1, kappa isotype control dashed line, Cat. No. 557721 or Alexa Fluor 488 Mouse anti-Sox2 monoclonal antibody solid line . Histograms were derived from gated events based on light scattering characteristics of the H9 cell line. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Analysis of Sox2 on human ES cell-derived neural stem cells NSC . NSC derived from H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 , and stained with matching concentrations of either Alexa Fluor 488 Mouse IgG1, kappa isotype control dashed line, Cat. No. 557721 or Alexa Fluor 488 Mouse Anti-Sox2 monoclonal antibody solid line . Histograms were derived from gated events based on light scattering characteristics of the H9-derived NSC. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Immunoflourescent staining of human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI , passage 33 grown on irradiated mouse embryonic fibroblasts, were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat No. 558050 , and stained with Alexa Fluor 488 Mouse anti-Sox2 monoclonal antibody pseudo colored green at 1.2 mug/mL. Counter-staining of cell nuclei was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Show More Analysis of Sox2 on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 and stained with matching concentrations of either Alexa Fluor 488 Mouse IgG1, kappa isotype control dashed line, Cat. No. 557721 or Alexa Fluor 488 Mouse anti-Sox2 monoclonal antibody solid line . Histograms were derived from gated events based on light scattering characteristics of the H9 cell line. Flow cytometry was performed on a BD LSR II flow cytometry system. Analysis of Sox2 on human ES cell-derived neural stem cells NSC . NSC derived from H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 , and stained with matching concentrations of either Alexa Fluor 488 Mouse IgG1, kappa isotype control dashed line, Cat. No. 557721 or Alexa Fluor 488 Mouse Anti-Sox2 monoclonal antibody solid line . Histograms were derived from gated events based on light scattering characteristics of the H9-derived NSC. Flow cytometry was performed on a BD LSR II flow cytometry system. Immunoflourescent staining of human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI , passage 33 grown on irradiated mouse embryonic fibroblasts, were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat No. 558050 , and stained with Alexa Fluor 488 Mouse anti-Sox2 monoclonal antibody pseudo colored green at 1.2 mug/mL. Counter-staining of cell nuclei was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software.