BD PharmingenTM Alexa Fluor 488 Mouse IgG1 kappa Isotype Control

Description

Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 , Cells were harvested, fixed, permeabilized and stained with PE-conjugated mouse anti-human CD8 PE-RPA-T8, Cat. No. 555367 and either mouse anti-human TNF antibody Alexa Fluor 488-MAb11, Cat. No. 557722 , left panel or Alexa Fluor 488-MOPC-21 immunoglobulin Cat. No. 557721 right panel by using the staining protocol found in the protocols section at www.bdbioscience.com. To deomonstrate specificty of staining the binding of Alexa Fluor 488-MAb11 was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF Cat. No. 554618 and by preincubation of the fixed permeabilized cells with an excess of unlabelled MAb11 antibody 5 mug, Cat. No. 554510 prior to staining. Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 , Cells were harvested, fixed, permeabilized and stained with PE-conjugated mouse anti-human CD8 PE-RPA-T8, Cat. No. 555367 and either mouse anti-human TNF antibody Alexa Fluor 488-MAb11, Cat. No. 557722 , left panel or Alexa Fluor 488-MOPC-21 immunoglobulin Cat. No. 557721 right panel by using the staining protocol found in the protocols section at www.bdbioscience.com. To deomonstrate specificty of staining the binding of Alexa Fluor 488-MAb11 was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF Cat. No. 554618 and by preincubation of the fixed permeabilized cells with an excess of unlabelled MAb11 antibody 5 mug, Cat. No. 554510 prior to staining. Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 , Cells were harvested, fixed, permeabilized and stained with PE-conjugated mouse anti-human CD8 PE-RPA-T8, Cat. No. 555367 and either mouse anti-human TNF antibody Alexa Fluor 488-MAb11, Cat. No. 557722 , left panel or Alexa Fluor 488-MOPC-21 immunoglobulin Cat. No. 557721 right panel by using the staining protocol found in the protocols section at www.bdbioscience.com. To deomonstrate specificty of staining the binding of Alexa Fluor 488-MAb11 was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF Cat. No. 554618 and by preincubation of the fixed permeabilized cells with an excess of unlabelled MAb11 antibody 5 mug, Cat. No. 554510 prior to staining. Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Show More Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 , Cells were harvested, fixed, permeabilized and stained with PE-conjugated mouse anti-human CD8 PE-RPA-T8, Cat. No. 555367 and either mouse anti-human TNF antibody Alexa Fluor 488-MAb11, Cat. No. 557722 , left panel or Alexa Fluor 488-MOPC-21 immunoglobulin Cat. No. 557721 right panel by using the staining protocol found in the protocols section at www.bdbioscience.com. To deomonstrate specificty of staining the binding of Alexa Fluor 488-MAb11 was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF Cat. No. 554618 and by preincubation of the fixed permeabilized cells with an excess of unlabelled MAb11 antibody 5 mug, Cat. No. 554510 prior to staining. Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Show More Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 , Cells were harvested, fixed, permeabilized and stained with PE-conjugated mouse anti-human CD8 PE-RPA-T8, Cat. No. 555367 and either mouse anti-human TNF antibody Alexa Fluor 488-MAb11, Cat. No. 557722 , left panel or Alexa Fluor 488-MOPC-21 immunoglobulin Cat. No. 557721 right panel by using the staining protocol found in the protocols section at www.bdbioscience.com. To deomonstrate specificty of staining the binding of Alexa Fluor 488-MAb11 was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF Cat. No. 554618 and by preincubation of the fixed permeabilized cells with an excess of unlabelled MAb11 antibody 5 mug, Cat. No. 554510 prior to staining. Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 , Cells were harvested, fixed, permeabilized and stained with PE-conjugated mouse anti-human CD8 PE-RPA-T8, Cat. No. 555367 and either mouse anti-human TNF antibody Alexa Fluor 488-MAb11, Cat. No. 557722 , left panel or Alexa Fluor 488-MOPC-21 immunoglobulin Cat. No. 557721 right panel by using the staining protocol found in the protocols section at www.bdbioscience.com. To deomonstrate specificty of staining the binding of Alexa Fluor 488-MAb11 was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF Cat. No. 554618 and by preincubation of the fixed permeabilized cells with an excess of unlabelled MAb11 antibody 5 mug, Cat. No. 554510 prior to staining. Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls.