BD PharmingenTM Alexa Fluor 488 Rat Anti-Mouse CD147

Description

Flow cytometric analysis of CD147 expression on BALB/c mouse thymocytes. BALB/c thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; dashed line histogram or Alexa Fluor 488 Rat Anti-Mouse CD147 antibody Cat. No. 566869; solid line histogram at 1 mug/ml. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD147 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD147 expression on BALB/c mouse thymocytes. BALB/c thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; dashed line histogram or Alexa Fluor 488 Rat Anti-Mouse CD147 antibody Cat. No. 566869; solid line histogram at 1 mug/ml. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD147 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD147 expression on BALB/c mouse thymocytes. BALB/c thymocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; dashed line histogram or Alexa Fluor 488 Rat Anti-Mouse CD147 antibody Cat. No. 566869; solid line histogram at 1 mug/ml. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD147 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative thymocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.