BD PharmingenTM Alexa Fluor 488 Rat Anti-Mouse CD4

Description

Immunohistofluorescent analysis of CD4 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with Alexa Fluor 488 Rat Anti-Mouse CD4 antibody Cat. No. 557667, pseudo-colored red , BD Horizon BV421 Rat Anti-Mouse CD8a antibody Cat. No. 563898, pseudo-colored green , and Alexa Fluor 647 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 557683, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Immunohistofluorescent analysis of CD4 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with Alexa Fluor 488 Rat Anti-Mouse CD4 antibody Cat. No. 557667, pseudo-colored red , BD Horizon BV421 Rat Anti-Mouse CD8a antibody Cat. No. 563898, pseudo-colored green , and Alexa Fluor 647 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 557683, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Show More Immunohistofluorescent analysis of CD4 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with Alexa Fluor 488 Rat Anti-Mouse CD4 antibody Cat. No. 557667, pseudo-colored red , BD Horizon BV421 Rat Anti-Mouse CD8a antibody Cat. No. 563898, pseudo-colored green , and Alexa Fluor 647 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 557683, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x.