BD PharmingenTM Alexa Fluor 488 Rat Anti-Mouse Foxp3

Description

Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Balb/c splenic leucocytes were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 740007 and either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; Left Plot or Alexa Fluor 488 Rat Anti-Mouse Foxp3 Cat. No. 563487 antibodies at 0.5 mug/test. The bivariate pseudocolor density plot shows the correlated expression patterns of CD4 versus Foxp3 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Balb/c splenic leucocytes were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 740007 and either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; Left Plot or Alexa Fluor 488 Rat Anti-Mouse Foxp3 Cat. No. 563487 antibodies at 0.5 mug/test. The bivariate pseudocolor density plot shows the correlated expression patterns of CD4 versus Foxp3 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Balb/c splenic leucocytes were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 740007 and either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; Left Plot or Alexa Fluor 488 Rat Anti-Mouse Foxp3 Cat. No. 563487 antibodies at 0.5 mug/test. The bivariate pseudocolor density plot shows the correlated expression patterns of CD4 versus Foxp3 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Show More Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Balb/c splenic leucocytes were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 740007 and either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; Left Plot or Alexa Fluor 488 Rat Anti-Mouse Foxp3 Cat. No. 563487 antibodies at 0.5 mug/test. The bivariate pseudocolor density plot shows the correlated expression patterns of CD4 versus Foxp3 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Show More Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Balb/c splenic leucocytes were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 740007 and either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; Left Plot or Alexa Fluor 488 Rat Anti-Mouse Foxp3 Cat. No. 563487 antibodies at 0.5 mug/test. The bivariate pseudocolor density plot shows the correlated expression patterns of CD4 versus Foxp3 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenocytes. Balb/c splenic leucocytes were fixed and permeabilized using the Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse CD4 Cat. No. 740007 and either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; Left Plot or Alexa Fluor 488 Rat Anti-Mouse Foxp3 Cat. No. 563487 antibodies at 0.5 mug/test. The bivariate pseudocolor density plot shows the correlated expression patterns of CD4 versus Foxp3 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software.