BD PharmingenTM Alexa Fluor 488 Rat anti-Mouse IL-17A

Description

Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated left and middle panels or unstimulated right panel with PMA 50 ng/ml final concentration; Sigma, Cat. No.P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No.I-0634 in the presence of BD GolgiStop Cat. No. 554724 . The cells were fixed, permeabilized and subsequently stained with Alexa 488 conjugated anti-mouse IL-17A clone TC11-18H10, Cat. No. 560220/560221 middle and right panels or Alexa 488 rat IgG1 isotype control clone R3-34, Cat. no. 557720 left panel using Pharmingens I/C staining protocol. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained right panel and non-specific antibody left panel controls . Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated left and middle panels or unstimulated right panel with PMA 50 ng/ml final concentration; Sigma, Cat. No.P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No.I-0634 in the presence of BD GolgiStop Cat. No. 554724 . The cells were fixed, permeabilized and subsequently stained with Alexa 488 conjugated anti-mouse IL-17A clone TC11-18H10, Cat. No. 560220/560221 middle and right panels or Alexa 488 rat IgG1 isotype control clone R3-34, Cat. no. 557720 left panel using Pharmingens I/C staining protocol. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained right panel and non-specific antibody left panel controls . Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated left and middle panels or unstimulated right panel with PMA 50 ng/ml final concentration; Sigma, Cat. No.P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No.I-0634 in the presence of BD GolgiStop Cat. No. 554724 . The cells were fixed, permeabilized and subsequently stained with Alexa 488 conjugated anti-mouse IL-17A clone TC11-18H10, Cat. No. 560220/560221 middle and right panels or Alexa 488 rat IgG1 isotype control clone R3-34, Cat. no. 557720 left panel using Pharmingens I/C staining protocol. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained right panel and non-specific antibody left panel controls . Show More Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated left and middle panels or unstimulated right panel with PMA 50 ng/ml final concentration; Sigma, Cat. No.P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No.I-0634 in the presence of BD GolgiStop Cat. No. 554724 . The cells were fixed, permeabilized and subsequently stained with Alexa 488 conjugated anti-mouse IL-17A clone TC11-18H10, Cat. No. 560220/560221 middle and right panels or Alexa 488 rat IgG1 isotype control clone R3-34, Cat. no. 557720 left panel using Pharmingens I/C staining protocol. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained right panel and non-specific antibody left panel controls . Show More Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated left and middle panels or unstimulated right panel with PMA 50 ng/ml final concentration; Sigma, Cat. No.P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No.I-0634 in the presence of BD GolgiStop Cat. No. 554724 . The cells were fixed, permeabilized and subsequently stained with Alexa 488 conjugated anti-mouse IL-17A clone TC11-18H10, Cat. No. 560220/560221 middle and right panels or Alexa 488 rat IgG1 isotype control clone R3-34, Cat. no. 557720 left panel using Pharmingens I/C staining protocol. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained right panel and non-specific antibody left panel controls . Characterization of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated left and middle panels or unstimulated right panel with PMA 50 ng/ml final concentration; Sigma, Cat. No.P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No.I-0634 in the presence of BD GolgiStop Cat. No. 554724 . The cells were fixed, permeabilized and subsequently stained with Alexa 488 conjugated anti-mouse IL-17A clone TC11-18H10, Cat. No. 560220/560221 middle and right panels or Alexa 488 rat IgG1 isotype control clone R3-34, Cat. no. 557720 left panel using Pharmingens I/C staining protocol. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the unstimulated stained right panel and non-specific antibody left panel controls .