BD PharmingenTM Alexa Fluor 488 Rat IgG2b, kappa Isotype Control

Description

Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of BD GolgiPlug Cat. No. 555029 . Cells were harvested, fixed, permeabilized, and stained with PE-conjugated rat anti-mouse CD4 PE-RM4-5, Cat. No. 553048 and either rat anti-mouse IL-2 antibody Alexa Fluor 488-JES6-5H4, Cat. No. 557725 , left panel or immunoglobulin isotype control Alexa Fluor 488-A95-1, Cat. No. 557726 , right panel by using Pharmingens staining protocol. Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of BD GolgiPlug Cat. No. 555029 . Cells were harvested, fixed, permeabilized, and stained with PE-conjugated rat anti-mouse CD4 PE-RM4-5, Cat. No. 553048 and either rat anti-mouse IL-2 antibody Alexa Fluor 488-JES6-5H4, Cat. No. 557725 , left panel or immunoglobulin isotype control Alexa Fluor 488-A95-1, Cat. No. 557726 , right panel by using Pharmingens staining protocol. Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of BD GolgiPlug Cat. No. 555029 . Cells were harvested, fixed, permeabilized, and stained with PE-conjugated rat anti-mouse CD4 PE-RM4-5, Cat. No. 553048 and either rat anti-mouse IL-2 antibody Alexa Fluor 488-JES6-5H4, Cat. No. 557725 , left panel or immunoglobulin isotype control Alexa Fluor 488-A95-1, Cat. No. 557726 , right panel by using Pharmingens staining protocol. Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Show More Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of BD GolgiPlug Cat. No. 555029 . Cells were harvested, fixed, permeabilized, and stained with PE-conjugated rat anti-mouse CD4 PE-RM4-5, Cat. No. 553048 and either rat anti-mouse IL-2 antibody Alexa Fluor 488-JES6-5H4, Cat. No. 557725 , left panel or immunoglobulin isotype control Alexa Fluor 488-A95-1, Cat. No. 557726 , right panel by using Pharmingens staining protocol. Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Show More Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of BD GolgiPlug Cat. No. 555029 . Cells were harvested, fixed, permeabilized, and stained with PE-conjugated rat anti-mouse CD4 PE-RM4-5, Cat. No. 553048 and either rat anti-mouse IL-2 antibody Alexa Fluor 488-JES6-5H4, Cat. No. 557725 , left panel or immunoglobulin isotype control Alexa Fluor 488-A95-1, Cat. No. 557726 , right panel by using Pharmingens staining protocol. Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of BD GolgiPlug Cat. No. 555029 . Cells were harvested, fixed, permeabilized, and stained with PE-conjugated rat anti-mouse CD4 PE-RM4-5, Cat. No. 553048 and either rat anti-mouse IL-2 antibody Alexa Fluor 488-JES6-5H4, Cat. No. 557725 , left panel or immunoglobulin isotype control Alexa Fluor 488-A95-1, Cat. No. 557726 , right panel by using Pharmingens staining protocol. Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls.