BD PharmingenTM Alexa Fluor 555 Mouse anti-Oct3/4

Description

Immunofluorescent staining of human ES cell line. H9 cells WiCell, Madison, WI were grown on mouse epithelial feeder cells in a BD Falcon 96-well Imaging Plate Cat. No. 353219 . After overnight incubation, the cells were fixed, permeabilized with BD Perm/Wash Buffer Cat No. 554723 , stained with Alexa Fluor 555 Mouse anti-Oct 3/4 pseudo colored red , and counter stained with Hoechst 33342 pseudo colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 High-Content Bioimager System with a 20x objective and merged using BD AttoVision software. This antibody also stained human EC NCCIT ATCC, Cat. No. CRL-2073 , mouse EC F9 ATCC, Cat. No. CRL-1720 , and mouse ES-E14TG2a ATCC, Cat. No. CRL-1821 cells. It also works with Triton X-100 and methanol permeabilization see Recommended Assay Procedure . Immunofluorescent staining of human ES cell line. H9 cells WiCell, Madison, WI were grown on mouse epithelial feeder cells in a BD Falcon 96-well Imaging Plate Cat. No. 353219 . After overnight incubation, the cells were fixed, permeabilized with BD Perm/Wash Buffer Cat No. 554723 , stained with Alexa Fluor 555 Mouse anti-Oct 3/4 pseudo colored red , and counter stained with Hoechst 33342 pseudo colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 High-Content Bioimager System with a 20x objective and merged using BD AttoVision software. This antibody also stained human EC NCCIT ATCC, Cat. No. CRL-2073 , mouse EC F9 ATCC, Cat. No. CRL-1720 , and mouse ES-E14TG2a ATCC, Cat. No. CRL-1821 cells. It also works with Triton X-100 and methanol permeabilization see Recommended Assay Procedure . Show More Immunofluorescent staining of human ES cell line. H9 cells WiCell, Madison, WI were grown on mouse epithelial feeder cells in a BD Falcon 96-well Imaging Plate Cat. No. 353219 . After overnight incubation, the cells were fixed, permeabilized with BD Perm/Wash Buffer Cat No. 554723 , stained with Alexa Fluor 555 Mouse anti-Oct 3/4 pseudo colored red , and counter stained with Hoechst 33342 pseudo colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 435 High-Content Bioimager System with a 20x objective and merged using BD AttoVision software. This antibody also stained human EC NCCIT ATCC, Cat. No. CRL-2073 , mouse EC F9 ATCC, Cat. No. CRL-1720 , and mouse ES-E14TG2a ATCC, Cat. No. CRL-1821 cells. It also works with Triton X-100 and methanol permeabilization see Recommended Assay Procedure .