BD PharmingenTM Alexa Fluor 647 Mouse Anti-C-Peptide

Description

Flow cytometric analysis of C-Peptide expression in human INS-transfected 293F cells and a mouse insulinoma cell line. LEFT: Untransfected dashed-line histogram and human INS-transfected solid-line histogram 293F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with Alexa Fluor 647 Mouse Anti-C-Peptide Cat. No. 565831 . RIGHT: Beta-TC-6 cells ATCC CRL-11505 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and stained with either Alexa Fluor 647 Mouse IgG1 kappa Isotype control Cat. No. 557783, dashed-line histogram or Alexa Fluor 647 Mouse Anti-C-Peptide Cat. No. 565831, solid-line histogram . All fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Immunohistofluorescent staining of C-Peptide in human, rat, and mouse islets of Langerhans. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , sections from formalin-fixed, paraffin-embedded human top panel , rat center panel , and mouse bottom panel pancreata were blocked using an Avidin/ Biotin Blocking Kit Vector Laboratories, Cat. No. SP-2001 as recommended by the manufacturer. The sections were then stained with Alexa Fluor 647 Mouse Anti-C-Peptide Cat. No. 565831, pseudocolored green . Cell nuclei were counterstained with DAPI Cat. No. 564907, pseudocolored red . The photographs were performed on a standard epifluorescence microscope. Original magnifications: 20X. Flow cytometric analysis of C-Peptide expression in human INS-transfected 293F cells and a mouse insulinoma cell line. LEFT: Untransfected dashed-line histogram and human INS-transfected solid-line histogram 293F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with Alexa Fluor 647 Mouse Anti-C-Peptide Cat. No. 565831 . RIGHT: Beta-TC-6 cells ATCC CRL-11505 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and stained with either Alexa Fluor 647 Mouse IgG1 kappa Isotype control Cat. No. 557783, dashed-line histogram or Alexa Fluor 647 Mouse Anti-C-Peptide Cat. No. 565831, solid-line histogram . All fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Show More Immunohistofluorescent staining of C-Peptide in human, rat, and mouse islets of Langerhans. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , sections from formalin-fixed, paraffin-embedded human top panel , rat center panel , and mouse bottom panel pancreata were blocked using an Avidin/ Biotin Blocking Kit Vector Laboratories, Cat. No. SP-2001 as recommended by the manufacturer. The sections were then stained with Alexa Fluor 647 Mouse Anti-C-Peptide Cat. No. 565831, pseudocolored green . Cell nuclei were counterstained with DAPI Cat. No. 564907, pseudocolored red . The photographs were performed on a standard epifluorescence microscope. Original magnifications: 20X. Show More Flow cytometric analysis of C-Peptide expression in human INS-transfected 293F cells and a mouse insulinoma cell line. LEFT: Untransfected dashed-line histogram and human INS-transfected solid-line histogram 293F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with Alexa Fluor 647 Mouse Anti-C-Peptide Cat. No. 565831 . RIGHT: Beta-TC-6 cells ATCC CRL-11505 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed and stained with either Alexa Fluor 647 Mouse IgG1 kappa Isotype control Cat. No. 557783, dashed-line histogram or Alexa Fluor 647 Mouse Anti-C-Peptide Cat. No. 565831, solid-line histogram . All fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Immunohistofluorescent staining of C-Peptide in human, rat, and mouse islets of Langerhans. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , sections from formalin-fixed, paraffin-embedded human top panel , rat center panel , and mouse bottom panel pancreata were blocked using an Avidin/ Biotin Blocking Kit Vector Laboratories, Cat. No. SP-2001 as recommended by the manufacturer. The sections were then stained with Alexa Fluor 647 Mouse Anti-C-Peptide Cat. No. 565831, pseudocolored green . Cell nuclei were counterstained with DAPI Cat. No. 564907, pseudocolored red . The photographs were performed on a standard epifluorescence microscope. Original magnifications: 20X.