BD PharmingenTM Alexa Fluor 647 Mouse anti-GATA3

Description

Comparison of GATA3 expression in human T and B cell lines. Jurkat T leukemia ATCC TIB152, shaded histogram and Ramos Burkitts lymphoma ATCC CRL-1596, open histogram were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for at least 30 minutes, and then stained with either Alexa Fluor 647 Mouse anti-GATA3 Cat. No. 560068/560078 or Alexa Fluor 647 Mouse IgG1 kappa Isotype control Cat. No. 557783, not shown . The GATA3 staining on the Jurkat cell line was significantly brighter than the isotype control on Jurkat cells, while the GATA3 staining on the Ramos cells coincided very closely to its isotype control data not shown . Thus, GATA3 expression was detected on the T cell line but not the B cell line. Flow cytometry was performed on a BD FACSArray bioanalyzer system. Comparison of GATA3 expression in mouse Th2 and Th1 cell lines. D10.G4.1 Th2 lymphoblasts ATCC TIB-224, shaded histogram and 2D6 Th1 clone Ahn et al, 1998, open histogram were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for at least 30 minutes, and then stained with either Alexa Fluor 647 Mouse anti-GATA3 or Alexa Fluor 647 Mouse IgG1 kappa Isotype control Cat. No. 557783, not shown . When compared to the respective isotype controls, the GATA3 staining on the D10.G4.1 cell line was significantly brighter than on the 2D6 cells. Flow cytometry was performed on a BD FACSArray bioanalyzer system. Comparison of GATA3 expression in human T and B cell lines. Jurkat T leukemia ATCC TIB152, shaded histogram and Ramos Burkitts lymphoma ATCC CRL-1596, open histogram were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for at least 30 minutes, and then stained with either Alexa Fluor 647 Mouse anti-GATA3 Cat. No. 560068/560078 or Alexa Fluor 647 Mouse IgG1 kappa Isotype control Cat. No. 557783, not shown . The GATA3 staining on the Jurkat cell line was significantly brighter than the isotype control on Jurkat cells, while the GATA3 staining on the Ramos cells coincided very closely to its isotype control data not shown . Thus, GATA3 expression was detected on the T cell line but not the B cell line. Flow cytometry was performed on a BD FACSArray bioanalyzer system. Show More Comparison of GATA3 expression in mouse Th2 and Th1 cell lines. D10.G4.1 Th2 lymphoblasts ATCC TIB-224, shaded histogram and 2D6 Th1 clone Ahn et al, 1998, open histogram were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for at least 30 minutes, and then stained with either Alexa Fluor 647 Mouse anti-GATA3 or Alexa Fluor 647 Mouse IgG1 kappa Isotype control Cat. No. 557783, not shown . When compared to the respective isotype controls, the GATA3 staining on the D10.G4.1 cell line was significantly brighter than on the 2D6 cells. Flow cytometry was performed on a BD FACSArray bioanalyzer system. Show More Comparison of GATA3 expression in human T and B cell lines. Jurkat T leukemia ATCC TIB152, shaded histogram and Ramos Burkitts lymphoma ATCC CRL-1596, open histogram were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for at least 30 minutes, and then stained with either Alexa Fluor 647 Mouse anti-GATA3 Cat. No. 560068/560078 or Alexa Fluor 647 Mouse IgG1 kappa Isotype control Cat. No. 557783, not shown . The GATA3 staining on the Jurkat cell line was significantly brighter than the isotype control on Jurkat cells, while the GATA3 staining on the Ramos cells coincided very closely to its isotype control data not shown . Thus, GATA3 expression was detected on the T cell line but not the B cell line. Flow cytometry was performed on a BD FACSArray bioanalyzer system. Comparison of GATA3 expression in mouse Th2 and Th1 cell lines. D10.G4.1 Th2 lymphoblasts ATCC TIB-224, shaded histogram and 2D6 Th1 clone Ahn et al, 1998, open histogram were fixed with pre-warmed BD Cytofix buffer Cat. No. 554655 for 10 minutes at 37oC, permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 on ice for at least 30 minutes, and then stained with either Alexa Fluor 647 Mouse anti-GATA3 or Alexa Fluor 647 Mouse IgG1 kappa Isotype control Cat. No. 557783, not shown . When compared to the respective isotype controls, the GATA3 staining on the D10.G4.1 cell line was significantly brighter than on the 2D6 cells. Flow cytometry was performed on a BD FACSArray bioanalyzer system.