BD PharmingenTM Alexa Fluor 647 Mouse Anti-GATA4

Description

Analysis of Alexa Fluor 647 Mouse Anti-GATA4 on mouse testis, embryonal carcinoma. F9 cells were treated with 0.3 mum Retinoic Acid and 1 mm Dibutyryl cAMP for 48 hours solid line or were untreated dashed line . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 oC, permeabilized with BD Perm Buffer III Cat. No. 558050 on ice for 30 minutes. The cells were stained with Alexa Fluor 647 Mouse Anti-GATA4, incubated in the dark for 30 minutes at room temperature. This antibody also works in BD Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Analysis of Alexa Fluor 647 Mouse Anti-GATA4 on mouse testis, embryonal carcinoma. F9 cells were treated with 0.3 mum Retinoic Acid and 1 mm Dibutyryl cAMP for 48 hours solid line or were untreated dashed line . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 oC, permeabilized with BD Perm Buffer III Cat. No. 558050 on ice for 30 minutes. The cells were stained with Alexa Fluor 647 Mouse Anti-GATA4, incubated in the dark for 30 minutes at room temperature. This antibody also works in BD Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Show More Analysis of Alexa Fluor 647 Mouse Anti-GATA4 on mouse testis, embryonal carcinoma. F9 cells were treated with 0.3 mum Retinoic Acid and 1 mm Dibutyryl cAMP for 48 hours solid line or were untreated dashed line . The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 10 minutes at 37 oC, permeabilized with BD Perm Buffer III Cat. No. 558050 on ice for 30 minutes. The cells were stained with Alexa Fluor 647 Mouse Anti-GATA4, incubated in the dark for 30 minutes at room temperature. This antibody also works in BD Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD FACSCanto II flow cytometry system.