BD PharmingenTM Alexa Fluor 647 Mouse anti-GFAP

Description

Immunofluorescent staining of Rat Brain left image . Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded sections were stained with Alexa Fluor 647 Mouse anti-GFAP monoclonal antibody pseudo colored magenta and counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System with a 20x objective and merged using BD Attovision software . Immunofluorescent staining of human grade III glioblastoma and astrocytoma cell line right image . U-373 cells ATCC HTB-17; discontinued, investigators may refer to: http://www.atcc.org/MisidentifiedCellLines/tabid/683/Default.aspx were seeded in a 96-well imaging plate Cat. No. 353219 at 15,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with cold methanol, and stained with Alexa Fluor 647 Mouse anti-GFAP pseudo colored magenta according to the Recommended Assay Procedure. Cell nuclei were counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System using a 20X objective and merged using BD AttoVision software. This antibody also stained GOS-3 cells DSMZ ACC 408; Human Astrocytoma; German Collection at http://www.dsmz.de/. It also worked with the Saponin and the Triton X-100 Perm/Wash protocols see Recommended Assay Procedure; Bioimaging protocol link . Immunofluorescent staining of Rat Brain left image . Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded sections were stained with Alexa Fluor 647 Mouse anti-GFAP monoclonal antibody pseudo colored magenta and counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System with a 20x objective and merged using BD Attovision software . Immunofluorescent staining of human grade III glioblastoma and astrocytoma cell line right image . U-373 cells ATCC HTB-17; discontinued, investigators may refer to: http://www.atcc.org/MisidentifiedCellLines/tabid/683/Default.aspx were seeded in a 96-well imaging plate Cat. No. 353219 at 15,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with cold methanol, and stained with Alexa Fluor 647 Mouse anti-GFAP pseudo colored magenta according to the Recommended Assay Procedure. Cell nuclei were counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System using a 20X objective and merged using BD AttoVision software. This antibody also stained GOS-3 cells DSMZ ACC 408; Human Astrocytoma; German Collection at http://www.dsmz.de/. It also worked with the Saponin and the Triton X-100 Perm/Wash protocols see Recommended Assay Procedure; Bioimaging protocol link . Immunofluorescent staining of Rat Brain left image . Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded sections were stained with Alexa Fluor 647 Mouse anti-GFAP monoclonal antibody pseudo colored magenta and counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System with a 20x objective and merged using BD Attovision software . Immunofluorescent staining of human grade III glioblastoma and astrocytoma cell line right image . U-373 cells ATCC HTB-17; discontinued, investigators may refer to: http://www.atcc.org/MisidentifiedCellLines/tabid/683/Default.aspx were seeded in a 96-well imaging plate Cat. No. 353219 at 15,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with cold methanol, and stained with Alexa Fluor 647 Mouse anti-GFAP pseudo colored magenta according to the Recommended Assay Procedure. Cell nuclei were counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System using a 20X objective and merged using BD AttoVision software. This antibody also stained GOS-3 cells DSMZ ACC 408; Human Astrocytoma; German Collection at http://www.dsmz.de/. It also worked with the Saponin and the Triton X-100 Perm/Wash protocols see Recommended Assay Procedure; Bioimaging protocol link . Show More Immunofluorescent staining of Rat Brain left image . Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded sections were stained with Alexa Fluor 647 Mouse anti-GFAP monoclonal antibody pseudo colored magenta and counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System with a 20x objective and merged using BD Attovision software . Immunofluorescent staining of human grade III glioblastoma and astrocytoma cell line right image . U-373 cells ATCC HTB-17; discontinued, investigators may refer to: http://www.atcc.org/MisidentifiedCellLines/tabid/683/Default.aspx were seeded in a 96-well imaging plate Cat. No. 353219 at 15,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with cold methanol, and stained with Alexa Fluor 647 Mouse anti-GFAP pseudo colored magenta according to the Recommended Assay Procedure. Cell nuclei were counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System using a 20X objective and merged using BD AttoVision software. This antibody also stained GOS-3 cells DSMZ ACC 408; Human Astrocytoma; German Collection at http://www.dsmz.de/. It also worked with the Saponin and the Triton X-100 Perm/Wash protocols see Recommended Assay Procedure; Bioimaging protocol link . Show More Immunofluorescent staining of Rat Brain left image . Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded sections were stained with Alexa Fluor 647 Mouse anti-GFAP monoclonal antibody pseudo colored magenta and counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System with a 20x objective and merged using BD Attovision software . Immunofluorescent staining of human grade III glioblastoma and astrocytoma cell line right image . U-373 cells ATCC HTB-17; discontinued, investigators may refer to: http://www.atcc.org/MisidentifiedCellLines/tabid/683/Default.aspx were seeded in a 96-well imaging plate Cat. No. 353219 at 15,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with cold methanol, and stained with Alexa Fluor 647 Mouse anti-GFAP pseudo colored magenta according to the Recommended Assay Procedure. Cell nuclei were counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System using a 20X objective and merged using BD AttoVision software. This antibody also stained GOS-3 cells DSMZ ACC 408; Human Astrocytoma; German Collection at http://www.dsmz.de/. It also worked with the Saponin and the Triton X-100 Perm/Wash protocols see Recommended Assay Procedure; Bioimaging protocol link . Immunofluorescent staining of Rat Brain left image . Following antigen retrieval with BD Retrievagen A buffer Cat. no. 550524 , the formalin-fixed paraffin-embedded sections were stained with Alexa Fluor 647 Mouse anti-GFAP monoclonal antibody pseudo colored magenta and counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System with a 20x objective and merged using BD Attovision software . Immunofluorescent staining of human grade III glioblastoma and astrocytoma cell line right image . U-373 cells ATCC HTB-17; discontinued, investigators may refer to: http://www.atcc.org/MisidentifiedCellLines/tabid/683/Default.aspx were seeded in a 96-well imaging plate Cat. No. 353219 at 15,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with cold methanol, and stained with Alexa Fluor 647 Mouse anti-GFAP pseudo colored magenta according to the Recommended Assay Procedure. Cell nuclei were counterstained with Hoechst 33342 pseudo colored blue . The images were captured on a BD Pathway 435 High-Content Bioimager System using a 20X objective and merged using BD AttoVision software. This antibody also stained GOS-3 cells DSMZ ACC 408; Human Astrocytoma; German Collection at http://www.dsmz.de/. It also worked with the Saponin and the Triton X-100 Perm/Wash protocols see Recommended Assay Procedure; Bioimaging protocol link .