BD PharmingenTM Alexa Fluor 647 Mouse anti-Hsp60

Description

Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor 647 Mouse anti-Hsp60 pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 855 Bioimager System with 20x left panel and 40X right panel objectives and merged using BD Attovision software. This antibody also stains A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells. Triton X-100 is not recommended as a permeabilization agent with this antibody conjugate. Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor 647 Mouse anti-Hsp60 pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 855 Bioimager System with 20x left panel and 40X right panel objectives and merged using BD Attovision software. This antibody also stains A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells. Triton X-100 is not recommended as a permeabilization agent with this antibody conjugate. Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor 647 Mouse anti-Hsp60 pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 855 Bioimager System with 20x left panel and 40X right panel objectives and merged using BD Attovision software. This antibody also stains A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells. Triton X-100 is not recommended as a permeabilization agent with this antibody conjugate. Show More Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor 647 Mouse anti-Hsp60 pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 855 Bioimager System with 20x left panel and 40X right panel objectives and merged using BD Attovision software. This antibody also stains A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells. Triton X-100 is not recommended as a permeabilization agent with this antibody conjugate. Show More Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor 647 Mouse anti-Hsp60 pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 855 Bioimager System with 20x left panel and 40X right panel objectives and merged using BD Attovision software. This antibody also stains A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells. Triton X-100 is not recommended as a permeabilization agent with this antibody conjugate. Immunofluorescent staining of human cell lines. HeLa cells ATCC CCL-2 were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor 647 Mouse anti-Hsp60 pseudo-colored green , and counter-stained with Hoechst 33342 pseudo-colored blue according to the Recommended Assay Procedure. The images were captured on a BD Pathway 855 Bioimager System with 20x left panel and 40X right panel objectives and merged using BD Attovision software. This antibody also stains A549 ATCC CCL-185 and U-2 OS ATCC HTB-96 cells. Triton X-100 is not recommended as a permeabilization agent with this antibody conjugate.