BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human CD100 (Sema4D)

Description

Multicolor flow cytometric analysis of CD100 expression on human peripheral blood cells. Human peripheral blood was treated with BD PharmLyse Lysing Buffer to lyse erythrocytes. The leucocytes were washed and stained with Alexa Fluor 488 Mouse Anti-Human CD4 Cat. No. 557695; Upper Panels and BD Horizon V450 Mouse Anti-Human CD19 Cat. No. 560353; Lower Panels antibodies, and with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat No. 557714; Left Panels or Alexa Fluor 647 Mouse Anti-Human CD100 Cat. No. 564873; Right Panels . The two-color flow cytometric contour plots showing the correlated expression of CD4 or CD19 versus CD100 or Ig Isotype control staining were derived ffrom gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System. Multicolor flow cytometric analysis of CD100 expression on human peripheral blood cells. Human peripheral blood was treated with BD PharmLyse Lysing Buffer to lyse erythrocytes. The leucocytes were washed and stained with Alexa Fluor 488 Mouse Anti-Human CD4 Cat. No. 557695; Upper Panels and BD Horizon V450 Mouse Anti-Human CD19 Cat. No. 560353; Lower Panels antibodies, and with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat No. 557714; Left Panels or Alexa Fluor 647 Mouse Anti-Human CD100 Cat. No. 564873; Right Panels . The two-color flow cytometric contour plots showing the correlated expression of CD4 or CD19 versus CD100 or Ig Isotype control staining were derived ffrom gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD100 expression on human peripheral blood cells. Human peripheral blood was treated with BD PharmLyse Lysing Buffer to lyse erythrocytes. The leucocytes were washed and stained with Alexa Fluor 488 Mouse Anti-Human CD4 Cat. No. 557695; Upper Panels and BD Horizon V450 Mouse Anti-Human CD19 Cat. No. 560353; Lower Panels antibodies, and with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat No. 557714; Left Panels or Alexa Fluor 647 Mouse Anti-Human CD100 Cat. No. 564873; Right Panels . The two-color flow cytometric contour plots showing the correlated expression of CD4 or CD19 versus CD100 or Ig Isotype control staining were derived ffrom gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometer System.