BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human CD116

Description

Two-parameter flow cytometric analysis of CD116 expression on human peripheral blood leucocytes. Human peripheral blood was treated with PharmLyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes prior to staining. The leucocytes were then preincubated with purified normal human polyclonal IgG 5 mug/106 cells and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714 or Alexa Fluor 647 Mouse Anti-Human CD116 antibody Cat No. 564046 . Two parameter flow cytometric contour plots showing the correlated expression of CD116 or Ig Isotype control staining versus side-light scatter SSC signals were derived from events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-parameter flow cytometric analysis of CD116 expression on human peripheral blood leucocytes. Human peripheral blood was treated with PharmLyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes prior to staining. The leucocytes were then preincubated with purified normal human polyclonal IgG 5 mug/106 cells and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714 or Alexa Fluor 647 Mouse Anti-Human CD116 antibody Cat No. 564046 . Two parameter flow cytometric contour plots showing the correlated expression of CD116 or Ig Isotype control staining versus side-light scatter SSC signals were derived from events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-parameter flow cytometric analysis of CD116 expression on human peripheral blood leucocytes. Human peripheral blood was treated with PharmLyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes prior to staining. The leucocytes were then preincubated with purified normal human polyclonal IgG 5 mug/106 cells and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714 or Alexa Fluor 647 Mouse Anti-Human CD116 antibody Cat No. 564046 . Two parameter flow cytometric contour plots showing the correlated expression of CD116 or Ig Isotype control staining versus side-light scatter SSC signals were derived from events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-parameter flow cytometric analysis of CD116 expression on human peripheral blood leucocytes. Human peripheral blood was treated with PharmLyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes prior to staining. The leucocytes were then preincubated with purified normal human polyclonal IgG 5 mug/106 cells and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714 or Alexa Fluor 647 Mouse Anti-Human CD116 antibody Cat No. 564046 . Two parameter flow cytometric contour plots showing the correlated expression of CD116 or Ig Isotype control staining versus side-light scatter SSC signals were derived from events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-parameter flow cytometric analysis of CD116 expression on human peripheral blood leucocytes. Human peripheral blood was treated with PharmLyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes prior to staining. The leucocytes were then preincubated with purified normal human polyclonal IgG 5 mug/106 cells and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714 or Alexa Fluor 647 Mouse Anti-Human CD116 antibody Cat No. 564046 . Two parameter flow cytometric contour plots showing the correlated expression of CD116 or Ig Isotype control staining versus side-light scatter SSC signals were derived from events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-parameter flow cytometric analysis of CD116 expression on human peripheral blood leucocytes. Human peripheral blood was treated with PharmLyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes prior to staining. The leucocytes were then preincubated with purified normal human polyclonal IgG 5 mug/106 cells and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714 or Alexa Fluor 647 Mouse Anti-Human CD116 antibody Cat No. 564046 . Two parameter flow cytometric contour plots showing the correlated expression of CD116 or Ig Isotype control staining versus side-light scatter SSC signals were derived from events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.